{"id":4123,"date":"2019-07-31T16:50:27","date_gmt":"2019-07-31T08:50:27","guid":{"rendered":"\/\/www.5080666.com\/product\/wsp-1-h2s-probe-%e7%a1%ab%e5%8c%96%e6%b0%a2%e8%8d%a7%e5%85%89%e6%8e%a2%e9%92%88\/"},"modified":"2024-06-06T10:48:36","modified_gmt":"2024-06-06T02:48:36","slug":"wsp-1-h2s-probe-%e7%a1%ab%e5%8c%96%e6%b0%a2%e8%8d%a7%e5%85%89%e6%8e%a2%e9%92%88","status":"publish","type":"product","link":"\/\/www.5080666.com\/product\/wsp-1-h2s-probe-%e7%a1%ab%e5%8c%96%e6%b0%a2%e8%8d%a7%e5%85%89%e6%8e%a2%e9%92%88\/","title":{"rendered":"WSP-1 (H2S Probe) \u786b\u5316\u6c22\u8367\u5149\u63a2\u9488"},"content":{"rendered":"

\u4ea7\u54c1\u63cf\u8ff0<\/strong><\/span><\/p>\n

WSP-1 (Washington State Probe-1)\u662f\u7531M. Xian\u56e2\u961f\u5f00\u53d1\u7684\u4e00\u79cd\u542b\u6d3b\u6027\u4e8c\u786b\u5316\u7269\u7684\u8367\u5149\u63a2\u9488\uff0c\u7279\u522b\u8bbe\u8ba1\u7528\u6765\u68c0\u6d4b\u751f\u7269\u6837\u672c\u548c\u7ec6\u80de\u5185\u7684\u786b\u5316\u6c22\uff08H2S\uff09\u3002\u901a\u8fc7\u57fa\u4e8e\u53cd\u5e94\u7684\u8367\u5149\u5f00\u542f\u7b56\u7565\uff0cWSP-1\u9009\u62e9\u6027\u4e14\u5feb\u901f\u4e0eH2S\u53cd\u5e94\uff0c\u751f\u6210\u82ef\u4e19\u4e8c\u786b\u9187\u916e\uff08benzodithiolone\uff09\u548c\u4e00\u79cd\u8367\u5149\u5316\u5408\u7269\uff08Ex=465nm\uff0cEm=515nm\uff09\uff08\u53cd\u5e94\u673a\u5236\u89c1\u4e0b\u56fe\uff09\u3002<\/span><\/p>\n

\"\"<\/span><\/p>\n

\u53c2\u8003\u6587\u732e\uff1aC. Liu, et al. Capture and Visualization of Hydrogen Sulfide via A Fluorescent Probe. Angew Chem Int Ed Engl. 2011 Oct 24; 50(44): 10327\u201310329.<\/span><\/p>\n

\u4ea7\u54c1\u6807\u7b7e<\/strong><\/span><\/p>\n

WSP-1 (Washington State Probe-1)\uff1bWSP-5\uff1b\u786b\u5316\u6c22\uff08H2S\uff09\u7279\u5f02\u6027\u63a2\u9488\uff1bPAG (DL-propargylglicine) \u786b\u5316\u6c22\u5408\u6210\u6291\u5236\u5242\uff1bHypotaurine\u00a0 H2S\u6e05\u9664\u5242\uff1bCAS NO\uff1a1352750-34-5\uff1b<\/span><\/p>\n

\u4ea7\u54c1\u4fe1\u606f<\/strong><\/span><\/p>\n\n\n\n\n\n
\u4ea7\u54c1\u540d\u79f0<\/span><\/td>\n\u4ea7\u54c1\u7f16\u53f7<\/span><\/td>\nCAS NO.<\/span><\/td>\n\u89c4\u683c<\/span><\/td>\n\u4ef7\u683c\uff08\u5143\uff09<\/span><\/td>\n<\/tr>\n
WSP-1 (H2S Probe) \u786b\u5316\u6c22\u8367\u5149\u63a2\u9488<\/span><\/td>\nMX5301-1MG<\/span><\/td>\n1352750-34-5<\/span><\/td>\n1mg<\/span><\/td>\n868<\/span><\/td>\n<\/tr>\n
WSP-1 (H2S Probe) \u786b\u5316\u6c22\u8367\u5149\u63a2\u9488<\/span><\/td>\nMX5301-5MG<\/span><\/td>\n1352750-34-5<\/span><\/td>\n5mg<\/span><\/td>\n2980<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u4ea7\u54c1\u7279\u6027<\/strong><\/span><\/p>\n

1\uff09\u00a0\u00a0 CAS NO\uff1a1352750-34-5<\/span><\/p>\n

2\uff09\u00a0\u00a0 \u5316\u5b66\u540d\uff1a3′-methoxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthen]-6′-yl 2-(pyridin-2-yldisulfanyl)benzoate<\/span><\/p>\n

3\uff09\u00a0\u00a0 \u540c\u4e49\u540d\uff1aWashington State Probe-1\uff1b\u786b\u5316\u6c22\u8367\u5149\u63a2\u9488\uff1bH2S\u8367\u5149\u63a2\u9488\uff1b<\/span><\/p>\n

4\uff09\u00a0\u00a0 \u5206\u5b50\u5f0f\uff1aC33H21NO6S2<\/span><\/p>\n

5\uff09\u00a0\u00a0 \u5206\u5b50\u91cf\uff1a591.7<\/span><\/p>\n

6\uff09\u00a0\u00a0 \u7eaf\u5ea6\uff1a\u226595%<\/span><\/p>\n

7\uff09\u00a0\u00a0 Ex\/Em\uff1a465\/515nm<\/span><\/p>\n

8\uff09\u00a0\u00a0 \u6eb6\u89e3\u6027\uff1a\u6eb6\u4e8eDMSO\uff08~2mg\/ml\uff09\u3001DMF\uff08~20mg\/ml\uff09<\/span><\/p>\n

9\uff09\u00a0\u00a0 \u5316\u5b66\u7ed3\u6784\u5f0f\uff1a\"\"<\/span><\/p>\n

\u4fdd\u5b58\u4e0e\u8fd0\u8f93\u65b9\u6cd5<\/strong><\/span><\/p>\n

\u4fdd\u5b58\uff1a-20\u00baC\u907f\u5149\u5e72\u71e5\u4fdd\u5b58\uff0c\u81f3\u5c112\u5e74\u6709\u6548\u3002<\/span><\/p>\n

\u8fd0\u8f93\uff1a\u51b0\u888b\u8fd0\u8f93\u3002<\/span><\/p>\n

\u4f7f\u7528\u65b9\u6cd5\u3010\u89c1\u9644\u5f55 \u4f7f\u7528\u5ef6\u4f38\u8d44\u6599\u9605\u8bfb\u3011<\/strong><\/span><\/p>\n

1\u3001\u00a0\u00a0\u50a8\u5b58\u6db2\u5236\u5907<\/span><\/strong><\/span><\/p>\n

\u5c06\u4f4e\u6e29\u4fdd\u5b58\u7684\u4ea7\u54c1\u4ece\u51b0\u7bb1\u53d6\u51fa\uff0c\u7f6e\u4e8e\u5ba4\u6e29\u56de\u6e29\u81f3\u5c1120min\u3002\u4f4e\u901f\u79bb\u5fc33-5min\u3002\u4e4b\u540e\u5f80\u74f6\u5185\u52a0\u5165\u9002\u91cf\u65e0\u6c34DMSO\u5145\u5206\u6eb6\u89e3\u914d\u62105mM\u50a8\u5b58\u6db2\uff08\u6bd4\u5982\uff0c1mg WSP-1\uff08Mw: 591.7\uff09\u52a0\u5165338\u03bcl DMSO\uff09\u3002\u6309\u7167\u5355\u6b21\u7528\u91cf\u5206\u88c5\u51bb\u5b58\uff0c\u907f\u514d\u53cd\u590d\u51bb\u878d\uff0c\u81f3\u5c113\u4e2a\u6708\u7a33\u5b9a\u3002<\/span><\/p>\n

2\u3001\u00a0\u00a0\u5de5\u4f5c\u6d53\u5ea6<\/span><\/strong><\/span><\/p>\n

\u6b63\u5f0f\u5b9e\u9a8c\u524d\uff0c\u53d65mM\u50a8\u5b58\u6db2\u7528\u9002\u5f53\u7f13\u51b2\u6db2\u6216\u57f9\u517b\u57fa\u7a00\u91ca\u5230\u6240\u9700\u5de5\u4f5c\u6d53\u5ea6\u5373\u53ef\u3002\u5177\u4f53\u7684\u5de5\u4f5c\u6d53\u5ea6\u8bf7\u53c2\u8003\u6587\u732e\u6216\u6839\u636e\u81ea\u8eab\u5b9e\u9a8c\u9700\u6c42\u6765\u8fdb\u884c\u4f18\u5316\u8c03\u6574\u3002\u53c2\u8003\u4e0b\u65b9\u4e00\u4e9b\u5b9e\u9a8c\u6570\u636e\uff0c\u6765\u81ea\u516c\u5f00\u6587\u732e\uff0c\u4ec5\u4f5c\u53c2\u8003\u3002<\/span><\/p>\n

\u5e94\u7528\u793a\u4f8b1\uff1a<\/span><\/strong>\u4f7f\u7528WSP-1\uff0810 \u00b5M\uff09\u68c0\u6d4bRFL-6\u7ec6\u80de\u5728\u786b\u5316\u7269\u4f9b\u4f53GYY4137\uff0810\u2013100 \u00b5M\uff09\u5185\u7684\u786b\u5316\u7269\u6c34\u5e73\uff0c\u7ecf\u6d41\u5f0f\u7ec6\u80de\u4eea\uff08FL1\u901a\u9053\uff09\u6765\u5b9a\u91cf\u5206\u6790\u3002\u6587\u732e\u6765\u6e90\uff1aCortese-Krott MM et al. Nitrosopersulfide (SSNO(-)) accounts for sustained NO bioactivity of S-nitrosothiols following reaction with sulfide. Redox Biol. 2014 Jan 11;2:234-44. PMID: 24494198.<\/span><\/p>\n

\u5e94\u7528\u793a\u4f8b2\uff1a<\/span><\/strong>\u4e3a\u4e86\u8bc1\u660eH2S\u5728NO\u8c03\u63a7\u7684\u4fa7\u6839\u5f62\u6210\u4e2d\u8d77\u5230\u7684\u91cd\u8981\u8c03\u63a7\u4f5c\u7528\uff0c\u4f7f\u7528WSP-1\u5bf9\u690d\u7269\u7ec6\u80de\u8fdb\u884c\u5185\u6e90\u6027H2S\u7684\u793a\u8e2a\u548c\u751f\u7269\u6210\u50cf\u68c0\u6d4b\u3002\u756a\u8304\u5e7c\u82d7\u6839\u90e8\u8f6c\u79fb\u542bWSP-1\uff0815 \u00b5M\uff09\u768420 mM Hepes-NaOH buffer\uff08pH 7.5\uff09\u300225\u2103\u907f\u5149\u5b75\u80b240min\uff0c\u6839\u90e8\u7528\u84b8\u998f\u6c34\u6e05\u6d17\u540e\u7acb\u5373\u7528\u8367\u5149\u663e\u5fae\u955c\u68c0\u6d4b\uff08Ex\/Em=465\/515nm\uff09\u3002\u6587\u732e\u6765\u6e90\uff1aLi YJ et al. In site bioimaging of hydrogen sulfide uncovers its pivotal role in regulating nitric oxide-induced lateral root formation. PLoS One. 2014 Feb 27;9(2):e90340. PMID: 24587333<\/span><\/p>\n

\u6ce8\u610f\u4e8b\u9879<\/strong><\/span><\/p>\n

1\uff09\u5bf9\u6211\u53f8\u63d0\u4f9b\u7684\u786b\u5316\u6c22\u63a2\u9488\uff08WSP-1\uff0cWSP-5\uff09\uff0c\u6211\u4eec\u53ea\u505a\u5316\u5408\u7269\u7eaf\u5ea6\u548c\u7ed3\u6784\u5b8c\u6574\u6027\u7684\u6d4b\u5b9a\uff0c\u5e76\u672a\u9488\u5bf9\u8fd9\u4e9b\u5316\u5408\u7269\u8fdb\u884c\u751f\u7269\u6d3b\u6027\u7684\u6d4b\u5b9a\u3002\u5173\u4e8e\u8fd9\u90e8\u5206\u7684\u4f7f\u7528\u6570\u636e\uff0c\u8bf7\u66f4\u591a\u53c2\u8003\u6587\u732e\u8d44\u6599\uff0c\u6216\u89c1\u6211\u53f8\u6574\u7406\u7684\u9644\u5f55 \u786b\u5316\u6c22\u63a2\u9488\uff08WSP-1\uff0cWSP-5\uff09\u4f7f\u7528\u5ef6\u4f38\u8d44\u6599\u9605\u8bfb\u3002<\/strong><\/span><\/p>\n

2\uff09\u4e3a\u4e86\u60a8\u7684\u5b89\u5168\u548c\u5065\u5eb7\uff0c\u8bf7\u7a7f\u5b9e\u9a8c\u670d\u5e76\u6234\u4e00\u6b21\u6027\u624b\u5957\u64cd\u4f5c\u3002<\/span><\/p>\n

\u76f8\u5173\u4ea7\u54c1<\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n
\u8d27\u53f7<\/span><\/td>\n\u540d\u79f0<\/span><\/td>\n\u89c4\u683c \u00a0 \u00a0 \u00a0 \u00a0 \u00a0\u00a0<\/span><\/td>\n<\/tr>\n
MX5301-1MG<\/span><\/td>\nWSP-1 (H2S Probe) \u786b\u5316\u6c22\u8367\u5149\u63a2\u9488<\/span><\/td>\n1mg<\/span><\/td>\n<\/tr>\n
MX5302-1MG<\/span><\/td>\nWSP-5 (H2S Probe) \u786b\u5316\u6c22\u8367\u5149\u63a2\u9488<\/span><\/td>\n1mg<\/span><\/td>\n<\/tr>\n
MX5305-50MG<\/span><\/td>\nHypotaurine \u4e9a\u725b\u78fa\u9178<\/span><\/td>\n50mg<\/span><\/td>\n<\/tr>\n
MX5306-250MG<\/span><\/td>\nDL-Propargylglycine DL-\u7094\u4e19\u57fa\u7518\u6c28\u9178<\/span><\/td>\n250mg<\/span><\/td>\n<\/tr>\n
MX5307-1MG<\/span><\/td>\nDL-Propargylglycine (hydrochloride) DL-\u7094\u4e19\u57fa\u7518\u6c28\u9178\u76d0\u9178\u76d0<\/span><\/td>\n1mg<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n

\u00a0\u2014 \u2014Written\/Edited by V. Shallan\u3010\u7248\u6743\u5f52\u96c6\u5947\u751f\u7269\/MKBio\u61cb\u5eb7\u6240\u6709\u3011<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

\u66f4\u591a\u4ea7\u54c1\u4fe1\u606f\u8bf7\u54a8\u8be2\u5ba2\u670d\uff01<\/p>\n","protected":false},"featured_media":28904,"template":"","meta":[],"product_cat":[99],"product_tag":[105],"_links":{"self":[{"href":"\/\/www.5080666.com\/wp-json\/wp\/v2\/product\/4123"}],"collection":[{"href":"\/\/www.5080666.com\/wp-json\/wp\/v2\/product"}],"about":[{"href":"\/\/www.5080666.com\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"\/\/www.5080666.com\/wp-json\/wp\/v2\/media\/28904"}],"wp:attachment":[{"href":"\/\/www.5080666.com\/wp-json\/wp\/v2\/media?parent=4123"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"\/\/www.5080666.com\/wp-json\/wp\/v2\/product_cat?post=4123"},{"taxonomy":"product_tag","embeddable":true,"href":"\/\/www.5080666.com\/wp-json\/wp\/v2\/product_tag?post=4123"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}