{"id":4095,"date":"2019-07-31T16:50:14","date_gmt":"2019-07-31T08:50:14","guid":{"rendered":"\/\/www.5080666.com\/product\/aminophenyl-fluorescein-apf-%e6%b0%a8%e5%9f%ba%e8%8b%af%e5%9f%ba%e8%8d%a7%e5%85%89%e7%b4%a0\/"},"modified":"2021-11-22T10:13:21","modified_gmt":"2021-11-22T02:13:21","slug":"aminophenyl-fluorescein-apf-%e6%b0%a8%e5%9f%ba%e8%8b%af%e5%9f%ba%e8%8d%a7%e5%85%89%e7%b4%a0","status":"publish","type":"product","link":"\/\/www.5080666.com\/product\/aminophenyl-fluorescein-apf-%e6%b0%a8%e5%9f%ba%e8%8b%af%e5%9f%ba%e8%8d%a7%e5%85%89%e7%b4%a0\/","title":{"rendered":"Aminophenyl fluorescein (APF) \u6c28\u57fa\u82ef\u57fa\u8367\u5149\u7d20"},"content":{"rendered":"

Aminophenyl fluorescein (APF<\/strong>) \u6c28\u57fa\u82ef\u57fa\u8367\u5149\u7d20<\/strong><\/span><\/p>\n

\u4ea7\u54c1\u4fe1\u606f<\/strong><\/span><\/p>\n

\n\n\n\n\n
\u00a0 \u4ea7\u54c1\u540d\u79f0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/span><\/td>\n\u00a0 \u4ea7\u54c1\u7f16\u53f7<\/span><\/td>\n\u00a0 \u89c4\u683c<\/span><\/td>\n\u00a0 \u4ef7\u683c\uff08\u5143\uff09<\/span><\/td>\n<\/tr>\n
\u00a0 Aminophenyl fluorescein (APF) \u6c28\u57fa\u82ef\u57fa\u8367\u5149\u7d20<\/span><\/td>\n\u00a0 MX4804-1MG<\/span><\/td>\n\u00a0 1mg<\/span><\/td>\n\u00a0 2180<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

 <\/p>\n

\u4ea7\u54c1\u63cf\u8ff0<\/strong><\/span><\/p>\n

\u6c28\u57fa\u82ef\u57fa\u8367\u5149\u7d20\uff08APF\uff09\u548c\u7f9f\u82ef\u57fa\u8367\u5149\u7d20\uff08HPF\uff09\u662f\u7531T. Nagano\u5f00\u53d1\u7684\u4e24\u79cd\u6d3b\u6027\u6c27\uff08Reactive oxygen species, ROS\uff09\u6307\u793a\u5242\uff0c\u6bd4H2DCFDA\uff08DCFH-DA, DCFH\uff09\u5177\u66f4\u4f18\u79c0\u7684\u7279\u5f02\u6027\u548c\u7a33\u5b9a\u6027\u3002APF\u548cHPF\u663e\u793a\u6709\u9650\u7684\u975e\u9009\u62e9\u6027\u53cd\u5e94\uff0c\u548c\u76f8\u5bf9\u8f83\u9ad8\u7684\u5149\u8bf1\u5bfc\u6c27\u5316\u6297\u6027\u3002APF\u548cHPF\u5177\u7ec6\u80de\u819c\u6e17\u900f\u6027\uff0c\u672c\u8eab\u65e0\u8367\u5149\uff0c\u76f4\u5230\u4e0e\u7f9f\u57fa\u81ea\u7531\u57fa\u6216\u8fc7\u6c27\u4e9a\u785d\u57fa\u9634\u79bb\u5b50\u53cd\u5e94\uff0c\u8fd8\u80fd\u4e0e\u6b21\u6c2f\u9178\u9634\u79bb\u5b50\u53cd\u5e94\uff0c\u4ea7\u751f\u5f3a\u7eff\u8272\u8367\u5149\uff08Ex\/Em\uff0c~490\/515nm\uff09\uff0c\u53ef\u7528\u8367\u5149\u663e\u5fae\u955c\u3001\u9ad8\u901a\u91cf\u6210\u50cf\u4eea\u3001\u8367\u5149\u9176\u6807\u677f\u6216\u6d41\u5f0f\u7ec6\u80de\u4eea\u68c0\u6d4b\u3002<\/span><\/p>\n

 <\/p>\n

\u672c\u54c1\u4ee5\u7c89\u672b\u5f62\u5f0f\u63d0\u4f9b\uff0c\u53ef\u76f4\u63a5\u6eb6\u4e8eDMF\u914d\u5236\u62105mM\u50a8\u5b58\u6db2\uff0c\u5e38\u7528\u5de5\u4f5c\u6d53\u5ea6\u8303\u56f41-10\u03bcM\uff0c\u9700\u6839\u636e\u81ea\u8eab\u7684\u5b9e\u9a8c\u4f53\u7cfb\u6216\u53c2\u8003\u6587\u732e\u8fdb\u884c\u4f18\u5316\u3002<\/span><\/p>\n

 <\/p>\n

\u4ea7\u54c1\u7279\u6027<\/strong><\/span><\/p>\n

1\uff09\u540c\u4e49\u540d\uff1a3′-(p-aminophenyl) fluorescein\u6c28\u57fa\u82ef\u57fa\u8367\u5149\u7d20<\/span><\/p>\n

2\uff09\u5206\u5b50\u5f0f\uff1aC26H17NO5<\/span><\/p>\n

3\uff09\u5206\u5b50\u91cf\uff1a423.42<\/span><\/p>\n

4\uff09\u7eaf\u5ea6\uff1a\u226595%<\/span><\/p>\n

5\uff09Ex\/Em\uff1a490\/515nm<\/span><\/p>\n

6\uff09\u6eb6\u89e3\u6027\uff1a\u6eb6\u4e8eDMSO\uff0810mM\uff09\u3001DMF\uff085mM\uff09<\/span><\/p>\n

7\uff09\u5316\u5b66\u7ed3\u6784\uff1a\"\"<\/span><\/p>\n

 <\/p>\n

\u4fdd\u5b58\u4e0e\u8fd0\u8f93\u65b9\u6cd5<\/strong><\/span><\/p>\n

\u4fdd\u5b58\uff1a-20\u00baC\u907f\u5149\u5e72\u71e5\u4fdd\u5b58\uff0c\u81f3\u5c111\u5e74\u6709\u6548\u3002<\/span><\/p>\n

\u8fd0\u8f93\uff1a\u51b0\u888b\u8fd0\u8f93\u3002<\/span><\/p>\n

 <\/p>\n

\u4f7f\u7528\u65b9\u6cd5 <\/strong><\/span><\/p>\n

\u4e00\u3001\u50a8\u5b58\u6db2\u5236\u5907<\/span><\/p>\n

\u5c06\u4f4e\u6e29\u4fdd\u5b58\u76841mg\u7c89\u672b\u7f6e\u4e8e\u5ba4\u6e29\u56de\u6e29\u81f3\u5c1120min\uff0c\u4e4b\u540e\u52a0\u5165472\u03bcl \u65e0\u6c34DMF\u914d\u7f6e\u62105mM\u50a8\u5b58\u6db2\u3002\u5145\u5206\u6eb6\u89e3\u548c\u6df7\u5300\u540e\uff0c\u6309\u7167\u5355\u6b21\u7528\u91cf\u5206\u88c5\uff0c-20\u00baC\u907f\u5149\u5e72\u71e5\u4fdd\u5b58\uff0c\u907f\u514d\u53cd\u590d\u51bb\u878d\u3002<\/span><\/p>\n

\u3010\u6ce8\u3011\uff1aAPF\u80fd\u6eb6\u4e8eDMSO\uff0810mM\uff09\uff0c\u4f46\u6709\u6570\u636e\u663e\u793aDMSO\u662f\u7f9f\u57fa\u81ea\u7531\u57fa\u7684\u6dec\u706d\u5242\uff0c\u4f1a\u5e72\u6270\u68c0\u6d4b\u548c\u964d\u4f4e\u7075\u654f\u6027\u3002\u56e0\u6b64\uff0c\u5efa\u8bae\u7528DMF\u4f5c\u4e3a\u6eb6\u5242\u3002<\/span><\/p>\n

\u4e8c\u3001\u6eb6\u6db2\u4f53\u7cfb\u68c0\u6d4b<\/span><\/p>\n

\u6b63\u5f0f\u5b9e\u9a8c\u524d\uff0c\u53d6\u4e00\u7ba1HPF\u50a8\u5b58\u6db2\u7f6e\u4e8e\u5ba4\u6e29\u56de\u6e29\uff0c\u4f7f\u5176\u5145\u5206\u878d\u89e3\u3002\u4e4b\u540e\u7528\u5408\u9002\u7684\u7f13\u51b2\u6db2\uff08\u6bd4\u5982HHBS\u7f13\u51b2\u6db2\uff0cPBS\u7f13\u51b2\u6db2\uff09\u7a00\u91ca\u5230\u6240\u9700\u7684\u5de5\u4f5c\u6d53\u5ea6\u3002\u5efa\u8bae\u8d77\u59cb\u6d53\u5ea6\u4e3a1-10\u03bcM\uff0c\u9700\u6839\u636e\u81ea\u8eab\u7684\u5b9e\u9a8c\u4f53\u7cfb\u6216\u53c2\u8003\u6587\u732e\u8fdb\u884c\u4f18\u5316\u3002<\/span><\/p>\n

\u4e09\u3001\u7ec6\u80de\u4f53\u7cfb\u68c0\u6d4b<\/span><\/p>\n

\u4ee5\u4e0b\u5b9e\u9a8c\u6b65\u9aa4\u4ec5\u505a\u53c2\u8003\uff0c\u6700\u4f73\u7684\u5de5\u4f5c\u6d53\u5ea6\u3001\u5b75\u80b2\u65f6\u95f4\u548c\u6e29\u5ea6\u9700\u6839\u636e\u5b9e\u9645\u60c5\u51b5\u8fdb\u884c\u8c03\u6574\u3002\u5728\u52a0\u5165APF\u5de5\u4f5c\u6db2\u4e4b\u524d\u6309\u9700\u5904\u7406\u7ec6\u80de\u3002<\/span><\/p>\n

1\uff09\u00a0\u00a0 \u6839\u636e\u5177\u4f53\u7528\u91cf\uff0c\u5c06DMF\u50a8\u5b58\u6db2\u7528HHBS\u7f13\u51b2\u6db2\u6216\u5176\u4ed6\u751f\u7406\u7f13\u51b2\u6db2\u7a00\u91ca\u62101-10\u03bcM\u5de5\u4f5c\u6db2\u3002<\/span><\/p>\n

2\uff09\u00a0\u00a0 \u6839\u636e\u5b9e\u9a8c\u8bbe\u8ba1\u5904\u7406\u7ec6\u80de\uff08\u6bd4\u5982\uff0cRASM\u7ec6\u80de\u752850-100nM \u8840\u7ba1\u7d27\u5f20\u7d20II\u5904\u74063-5h\uff09\u3002<\/span><\/p>\n

3\uff09\u00a0\u00a0 \u53d6\u6b65\u9aa41\uff09\u51c6\u5907\u76841-10\u03bcM\u5de5\u4f5c\u6db2\u52a0\u5165\u7ec6\u80de\uff0c37\u00baC\u5b75\u80b220-60min\u3002<\/span><\/p>\n

4\uff09\u00a0\u00a0 \u5438\u6389\u67d3\u8272\u5de5\u4f5c\u6db2\uff0c\u7528HHBS\u6216\u5176\u4ed6\u751f\u7406\u7f13\u51b2\u6db2\u66ff\u6362\u3002<\/span><\/p>\n

5\uff09\u00a0\u00a0 \u7528\u5408\u9002\u7684\u8367\u5149\u4eea\u5668\uff08Ex\/Em=492\/515nm\uff09\u6765\u68c0\u6d4b\u3002<\/span><\/p>\n

\u3010\u6ce81\u3011\uff1aBSA\u548c\u915a\u7ea2\u4f1a\u5f71\u54cd\u8367\u5149\uff0c\u9700\u8c28\u614e\u4f7f\u7528\u3002<\/span><\/p>\n

\u3010\u6ce82\u3011\uff1aHPF\u548cAPF\u80fd\u7528\u4e8e\u6eb6\u6db2\u4f53\u7cfb\u6216\u80de\u5185ROS\u68c0\u6d4b\u3002<\/span><\/p>\n

\u6ce8\u610f\u4e8b\u9879<\/strong><\/span><\/p>\n

1\uff09APF\u548cHPF\u4ec5\u7528\u4e8e\u79d1\u7814\u7814\u7a76\uff0c\u4e0d\u53ef\u7528\u4e8e\u4f53\u5916\u8bca\u65ad\u7814\u7a76\u3002<\/span><\/p>\n

2\uff09APF\u548cHPF\u4ea7\u751f\u7684\u8367\u5149\u76f8\u5bf9\u4e8e\u5176\u4ed6\u6d3b\u6027\u6c27\u63a2\u9488\u6bd4\u5982DCFH\u5f31\u5f88\u591a\uff0c\u8fd9\u662f\u63a2\u9488\u672c\u8eab\u7684\u7279\u6027\u51b3\u5b9a\u7684\uff08\u89c1\u9644\u88681 \u6d3b\u6027\u6c27\u63a2\u9488\uff08HPF, APF, DCFH\uff09\u5bf9\u5404\u79cd\u6d3b\u6027\u6c27\u7269\u8d28\u7684\u53cd\u5e94\u7279\u5f02\u6027\uff09\u3002<\/span><\/p>\n

3\uff09\u4e3a\u4e86\u60a8\u7684\u5b89\u5168\u548c\u5065\u5eb7\uff0c\u8bf7\u7a7f\u5b9e\u9a8c\u670d\u5e76\u6234\u4e00\u6b21\u6027\u624b\u5957\u64cd\u4f5c\u3002\u00a0<\/span><\/p>\n

 <\/p>\n

\u76f8\u5173\u4ea7\u54c1 <\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n
\u8d27\u53f7<\/span><\/td>\n\u540d\u79f0<\/span><\/td>\n\u89c4\u683c<\/span><\/td>\n<\/tr>\n
MX4801-1KIT<\/span><\/td>\nReactive Oxygen Species (ROS) Assay Kit \u6d3b \u6027\u6c27\uff08ROS\uff09\u68c0\u6d4b\u8bd5\u5242\u76d2<\/span><\/td>\n100~500T<\/span><\/td>\n<\/tr>\n
MX4802-50MG<\/span><\/td>\nH2DCFDA (DCFH-DA, DCFH) \u6d3b\u6027\u6c27\uff08ROS\uff09\u8367\u5149\u63a2\u9488<\/span><\/td>\n50mg<\/span><\/td>\n<\/tr>\n
MX4804-1MG<\/span><\/td>\nAminophenyl fluorescein (APF) \u6c28\u57fa\u82ef\u57fa\u8367\u5149\u7d20<\/span><\/td>\n1mg<\/span><\/td>\n<\/tr>\n
MX4805-1MG<\/span><\/td>\nHydroxyphenyl fluorescein (HPF) \u7f9f\u82ef\u57fa\u8367\u5149\u7d20<\/span><\/td>\n1mg<\/span><\/td>\n<\/tr>\n
MX4455-1MG<\/span><\/td>\nDihydrorhodamine 123 (DHR 123) \u4e8c\u6c22\u7f57\u4e39\u660e123<\/span><\/td>\n1mg<\/span><\/td>\n<\/tr>\n
MM0706-1G<\/span><\/td>\nLuminol \u9c81\u7c73\u8bfa<\/span><\/td>\n1g<\/span><\/td>\n<\/tr>\n
MX4606-500UG<\/span><\/td>\nCoelenterazine Native \u5929\u7136\u8154\u80a0\u7d20<\/span><\/td>\n500\u03bcg<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u9644\u88681 \u6d3b\u6027\u6c27\u63a2\u9488\uff08HPF, APF, DCFH\uff09\u5bf9\u5404\u79cd\u6d3b\u6027\u6c27\u7269\u8d28\u7684\u53cd\u5e94\u7279\u5f02\u6027 <\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
ROS<\/span><\/td>\nROS\u4ea7\u751f\u65b9\u6cd5<\/span><\/td>\nHPF (MX4805)<\/span><\/td>\nAPF (MX4804)<\/span><\/td>\nDCFH (MX4802)<\/span><\/td>\n<\/tr>\n
HO\u2022<\/span><\/td>\n100 \u00b5M\u9ad8\u6c2f\u9178\u4e9a\u94c1(II)+1 mMH2O2<\/span><\/td>\n730<\/span><\/td>\n1200<\/span><\/td>\n7400<\/span><\/td>\n<\/tr>\n
ONOO\u2013<\/span><\/td>\n3 \u00b5M (\u7ec8\u6d53\u5ea6) ONOO\u2013<\/span><\/td>\n120<\/span><\/td>\n560<\/span><\/td>\n6600<\/span><\/td>\n<\/tr>\n
OCl-<\/span><\/td>\n3 \u00b5M (\u7ec8\u6d53\u5ea6) OCl-<\/span><\/td>\n6<\/span><\/td>\n3600<\/span><\/td>\n86<\/span><\/td>\n<\/tr>\n
1O2<\/span><\/td>\n100 \u00b5M 3-(1,4-dihydro-1,4-epidioxy-1-naphthyl)<\/span><\/p>\n

propionic acid<\/span><\/td>\n

5<\/span><\/td>\n9<\/span><\/td>\n26<\/span><\/td>\n<\/tr>\n
\u2022O2\u2013<\/span><\/td>\n100 \u00b5M KO2<\/span><\/td>\n8<\/span><\/td>\n6<\/span><\/td>\n67<\/span><\/td>\n<\/tr>\n
H2O2<\/span><\/td>\n100 \u00b5M of H2O2<\/span><\/td>\n2<\/span><\/td>\n\uff1c1<\/span><\/td>\n190<\/span><\/td>\n<\/tr>\n
NO<\/span><\/td>\n100 \u00b5M NOC-7<\/span><\/td>\n6<\/span><\/td>\n\uff1c1<\/span><\/td>\n150<\/span><\/td>\n<\/tr>\n
ROO\u2022<\/span><\/td>\n100 \u00b5M AAPH<\/span><\/td>\n17<\/span><\/td>\n2<\/span><\/td>\n710<\/span><\/td>\n<\/tr>\n
Autoxidation<\/span><\/td>\n\u8367\u5149\u5149\u71672.5h<\/span><\/td>\n\uff1c1<\/span><\/td>\n\uff1c1<\/span><\/td>\n2000<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0 <\/strong><\/span><\/p>\n

\u00a0 <\/strong><\/span><\/p>\n

\u00a0\u2014 \u2014Written\/Edited by V. Shallan\u3010\u7248\u6743\u5f52\u96c6\u5947\u751f\u7269\/MKBio\u61cb\u5eb7\u6240\u6709\u3011<\/span><\/p>\n

 <\/p>\n

\u6587\u732e\u5f15\u7528<\/span><\/strong><\/span><\/p>\n

\u30101\u3011<\/span>Wu Q et al. Cascade enzymes within self-assembled hybrid nanogel mimicked neutrophil lysosomes for singlet oxygen elevated cancer therapy. Nat Commun. 2019 Jan 16;10(1):240.<\/span><\/p>\n

 <\/p>\n

The aminophenyl fluorescein (APF) as the HClO detector were purchased from Shanghai Maokangbio. The further EDT related therapeutic species including the ROS, 1O2 and the intermediate HClO have been detected in tumour tissues of different groups by using the corresponding fluorescent probes, DCFH-DA, SOSG and aminophenyl fluorescein (APF, as HClO detector).<\/span><\/p>\n

 <\/p>\n

\u30102\u3011<\/span>Juan J et al. Dynamic tracking of bulk nanobubbles from microbubbles shrinkage to collapse. Colloids and Surfaces A: Physicochemical and Engineering Aspects. Volume 589, 20 February 2020, 124430<\/span><\/p>\n

3\u2032- p – (aminophenyl) fluorescein (APF) was purchased from Shanghai Maokang Biotechnology Co., Ltd. (shanghai, China).<\/span><\/p>\n

 <\/p>\n

[3]<\/span>Yahui Zhang, Weizhou Sha, Yang Liu, Wei Wang, Zhi Yuan. A facile composite nanoparticle promoted by photoelectron transfer and consumption for tumor combination therapy.\u00a0Mater. Chem. Front., 2020,4, 3047-3056 \u00a0https:\/\/doi.org\/10.1039\/D0QM00447B<\/u><\/a><\/span><\/p>\n

3\u2032-(p-aminophenyl) fluorescein (APF) was purchased from maokang-bio (Shang hai).<\/span><\/p>\n

 <\/p>\n

[4]<\/span>Li L, Shao C, Liu T, Chao Z, Chen H, Xiao F, He H, Wei Z, Zhu Y, Wang H, Zhang X, Wen Y, Yang B, He F, Tian L. An NIR-II-Emissive Photosensitizer for Hypoxia-Tolerant Photodynamic Theranostics. Adv Mater. 2020 Nov;32(45):e2003471. doi: 10.1002\/adma.202003471. Epub 2020 Oct 7. PMID: 33029855.<\/span><\/p>\n

2,7-Dichlorodi-hydrofluorescein diacetate (DCFH-DA), aminophenyl fluorescein (APF), and singlet oxygen sensor green (SOSG) were obtained from Shanghai Maokang Biotechnology Co., Ltd. (Shanghai, China).<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

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