{"id":3666,"date":"2019-07-31T16:47:07","date_gmt":"2019-07-31T08:47:07","guid":{"rendered":"\/\/www.5080666.com\/product\/annexin-v-fitc-pi-apoptosis-detection-kit-%e7%bb%86%e8%83%9e%e5%87%8b%e4%ba%a1%e6%a3%80%e6%b5%8b%e8%af%95%e5%89%82%e7%9b%92\/"},"modified":"2021-12-03T10:26:17","modified_gmt":"2021-12-03T02:26:17","slug":"annexin-v-fitc-pi-apoptosis-detection-kit-%e7%bb%86%e8%83%9e%e5%87%8b%e4%ba%a1%e6%a3%80%e6%b5%8b%e8%af%95%e5%89%82%e7%9b%92","status":"publish","type":"product","link":"\/\/www.5080666.com\/product\/annexin-v-fitc-pi-apoptosis-detection-kit-%e7%bb%86%e8%83%9e%e5%87%8b%e4%ba%a1%e6%a3%80%e6%b5%8b%e8%af%95%e5%89%82%e7%9b%92\/","title":{"rendered":"Annexin V-FITC\/PI Apoptosis Detection Kit \u7ec6\u80de\u51cb\u4ea1\u68c0\u6d4b\u8bd5\u5242\u76d2"},"content":{"rendered":"

Annexin V- FITC\/PI Apoptosis Detection Kit<\/strong><\/span><\/p>\n

\u7ec6\u80de\u51cb\u4ea1\u68c0\u6d4b\u8bd5\u5242\u76d2<\/strong><\/span><\/p>\n

\u4ea7\u54c1\u4fe1\u606f<\/strong><\/span><\/p>\n\n\n\n\n\n\n
\u4ea7\u54c1\u540d\u79f0<\/span><\/td>\n\u4ea7\u54c1\u7f16\u53f7<\/span><\/td>\n\u89c4\u683c<\/span><\/td>\n\u4ef7\u683c\uff08\u5143\uff09<\/span><\/td>\n<\/tr>\n
Annexin V-FITC\/PI Apoptosis Detection Kit<\/span><\/p>\n

\u7ec6\u80de\u51cb\u4ea1\u68c0\u6d4b\u8bd5\u5242\u76d2<\/span><\/td>\n

MX3210-20T<\/span><\/td>\n20T<\/span><\/td>\n650<\/span><\/td>\n<\/tr>\n
MX3210-50T<\/span><\/td>\n50T<\/span><\/td>\n1350<\/span><\/td>\n<\/tr>\n
MX3210-100T<\/span><\/td>\n100T<\/span><\/td>\n1980<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u4ea7\u54c1\u63cf\u8ff0<\/strong><\/span><\/p>\n

Annexin V-FITC\/PI\u7ec6\u80de\u51cb\u4ea1\u68c0\u6d4b\u8bd5\u5242\u76d2\uff08Annexin V-FITC\/PI Apoptosis Detection Kit\uff09\u662f\u4e00\u6b3e\u7528\u6765\u68c0\u6d4b\u7ec6\u80de\u51cb\u4ea1\u7684\u8bd5\u5242\u76d2\uff0c\u53ef\u4ee5\u7528\u6d41\u5f0f\u7ec6\u80de\u4eea\u6216\u8367\u5149\u663e\u5fae\u955c\u8fdb\u884c\u68c0\u6d4b\u3002\u672c\u8bd5\u5242\u76d2\u5305\u542bFITC\u6807\u8bb0\u7684Annexin V\uff0c\u7528\u6765\u68c0\u6d4b\u65e9\u671f\u7ec6\u80de\u51cb\u4ea1\uff1b\u4ee5\u53ca\u7898\u5316\u4e19\u5576\uff08PI\uff09\uff0c\u7528\u6765\u68c0\u6d4b\u574f\u6b7b\u7ec6\u80de\u3002<\/span><\/p>\n

\u672c\u8bd5\u5242\u76d2\u7684\u5de5\u4f5c\u539f\u7406\u5728\u4e8e\uff1a\u78f7\u8102\u9170\u4e1d\u6c28\u9178\uff08Phosphotidylserine\uff0cPS\uff09\u662f\u4e00\u79cd\u5e26\u8d1f\u7535\u8377\u7684\u78f7\u8102\u3002\u6b63\u5e38\u7ec6\u80de\u4e2d\uff0cPS\u4f4d\u4e8e\u7ec6\u80de\u819c\u5185\u9762\uff0c\u4f46\u5f53\u51cb\u4ea1\u5f00\u59cb\u540e\uff0cPS\u5728\u78f7\u8102\u53cc\u5206\u5b50\u5c42\u4e2d\u7684\u4e0d\u5bf9\u79f0\u5206\u5e03\u9010\u6e10\u6d88\u5931\u5e76\u8f6c\u79fb\u5230\u7ec6\u80de\u819c\u8868\u9762\uff0c\u4ece\u800c\u66b4\u9732\u5728\u7ec6\u80de\u5916\u73af\u5883\u4e2d\u3002\u819c\u8054\u86cb\u767dV\uff08Annexin V\uff09\u662f\u4e00\u79cd\u5206\u5b50\u91cf\u7ea635kDa\u7684\u9499\u79bb\u5b50\u4f9d\u8d56\u6027\u78f7\u8102\u7ed3\u5408\u86cb\u767d\uff0c\u80fd\u975e\u5e38\u5bb9\u6613\u7684\u7ed3\u5408PS\uff0c\u4e14\u5177\u6709\u9ad8\u5ea6\u7684\u4eb2\u548c\u6027\u3002\u56e0\u6b64\uff0c\u901a\u8fc7Annexin V\u4e0ePS\u7684\u7ed3\u5408\u4e0e\u5426\u6765\u5224\u65ad\u51cb\u4ea1\u53d1\u751f\u3002\u53e6\u5916\uff0cPI\u7684\u5f15\u5165\u80fd\u8f85\u52a9\u533a\u5206\u51cb\u4ea1\u65e9\u671f\u7ec6\u80de\u548c\u665a\u671f\u51cb\u4ea1\u6216\u574f\u6b7b\u7ec6\u80de\u3002PI\u4f5c\u4e3a\u4e00\u79cd\u6d3b\u7ec6\u80de\u6392\u65a5\u63a2\u9488\uff0c\u4e0d\u80fd\u7a7f\u900f\u5177\u6709\u5b8c\u6574\u7ec6\u80de\u819c\u7684\u6b63\u5e38\u7ec6\u80de\u6216\u51cb\u4ea1\u65e9\u671f\u7ec6\u80de\uff0c\u4f46\u80fd\u7a7f\u900f\u4e27\u5931\u7ec6\u80de\u819c\u5b8c\u6574\u6027\u7684\u51cb\u4ea1\u665a\u671f\u7ec6\u80de\u6216\u574f\u6b7b\u7ec6\u80de\uff0c\u4ece\u800c\u5c06\u7ec6\u80de\u6838\u67d3\u7ea2\u3002\u56e0\u6b64\uff0c\u5c06Annexin V-FITC\u4e0ePI\u8054\u5408\u4f7f\u7528\u65f6\uff0c\u5448\u73b0\u7ed3\u679c\u4e3a\uff1a\u6b63\u5e38\u7ec6\u80de\uff08Annexin V-FITC-\/PI-\uff09\uff0c\u65e9\u671f\u51cb\u4ea1\u7ec6\u80de\uff08Annexin V-FITC+\/PI-\uff09\uff1b\u665a\u671f\u51cb\u4ea1\u7ec6\u80de\u548c\u574f\u6b7b\u7ec6\u80de\u540c\u65f6\u5448\u73b0\u53cc\u9633\u6027\uff08Annexin V-FITC+\/PI+\uff09\u3002<\/span><\/p>\n

\u4ea7\u54c1\u7ec4\u6210<\/strong><\/span><\/p>\n\n\n\n\n\n\n\n
\u7ec4\u5206\u7f16\u53f7<\/span><\/td>\n\u7ec4\u5206\u540d\u79f0<\/span><\/td>\n\u4fdd\u5b58\u65b9\u6cd5<\/span><\/td>\n\u4ea7\u54c1\u7f16\u53f7\uff08\u89c4\u683c\uff09<\/span><\/td>\n<\/tr>\n
MX3210-20T<\/span><\/td>\nMX3210-50T<\/span><\/td>\nMX3210-100T<\/span><\/td>\n<\/tr>\n
MX3210-A<\/span><\/td>\nAnnexin V-FITC<\/span><\/td>\n2-8\u2103\u907f\u5149<\/span><\/td>\n100\u03bcl<\/span><\/td>\n250\u03bcl<\/span><\/td>\n500\u03bcl<\/span><\/td>\n<\/tr>\n
MX3210-B<\/span><\/td>\n\u7898\u5316\u4e19\u5576\u6eb6\u6db2<\/span><\/td>\n2-8\u2103\u907f\u5149<\/span><\/td>\n200\u03bcl<\/span><\/td>\n500\u03bcl<\/span><\/td>\n1ml<\/span><\/td>\n<\/tr>\n
MX3210-C<\/span><\/td>\n\u7ed3\u5408\u7f13\u51b2\u6db2\uff084\u00d7\uff09<\/span><\/td>\n2-8\u2103<\/span><\/td>\n4ml<\/span><\/td>\n10ml<\/span><\/td>\n20ml<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u4fdd\u5b58\u4e0e\u8fd0\u8f93\u65b9\u6cd5<\/span><\/h2>\n

\u4fdd\u5b58\uff1a2-8\u2103\u4fdd\u5b58\uff0c\u4e0d\u53ef\u51b0\u51bb\u3002\u5176\u4e2d\u7ec4\u5206A\uff0cB\u9700\u907f\u5149\u4fdd\u5b58\u30021\u5e74\u6709\u6548\u3002<\/span><\/p>\n

\u8fd0\u8f93\uff1a\u51b0\u888b\u8fd0\u8f93\u3002<\/span><\/p>\n

\u6ce8\u610f\u4e8b\u9879<\/strong><\/span><\/p>\n

1\uff09 \u7531\u4e8e\u7ec6\u80de\u51cb\u4ea1\u662f\u4e00\u4e2a\u5feb\u901f\u8fc7\u7a0b\uff0c\u67d3\u8272\u540e\u8bf7\u5c3d\u5feb\u68c0\u6d4b\uff0c\u65f6\u95f4\u8fc7\u957f\u4f1a\u5bfc\u81f4\u51cb\u4ea1\u6216\u574f\u6b7b\u7ec6\u80de\u6570\u91cf\u589e\u52a0\u3002<\/span><\/p>\n

2\uff09 \u5bf9\u4e8e\u8d34\u58c1\u7ec6\u80de\uff0c\u9700\u8981\u5f88\u597d\u638c\u63a7\u6d88\u5316\u6b65\u9aa4\u3002\u7ec6\u80de\u51cb\u4ea1\u8bf1\u5bfc\u65f6\u8d34\u58c1\u7ec6\u80de\u5982\u6709\u6f02\u6d6e\u7ec6\u80de\uff0c\u9700\u540c\u65f6\u6536\u96c6\u6f02\u6d6e\u7ec6\u80de\u548c\u8d34\u58c1\u7ec6\u80de\u5408\u5e76\u67d3\u8272\u3002\u5904\u7406\u8d34\u58c1\u7ec6\u80de\u65f6\u8981\u5c0f\u5fc3\uff0c\u5c3d\u91cf\u907f\u514d\u4eba\u4e3a\u9020\u6210\u7684\u7ec6\u80de\u635f\u4f24\u3002\u80f0\u9176\u6d88\u5316\u65f6\u95f4\u8fc7\u77ed\uff0c\u7ec6\u80de\u9700\u8981\u7528\u529b\u5439\u6253\u624d\u80fd\u8131\u843d\uff0c\u5bb9\u6613\u9020\u6210\u7ec6\u80de\u819c\u7684\u635f\u4f24\uff0c\u5bfc\u81f4PI\u6444\u5165\u8fc7\u591a\uff1b\u800c\u80f0\u9176\u6d88\u5316\u65f6\u95f4\u8fc7\u957f\uff0c\u7ec6\u80de\u819c\u4e5f\u6613\u9020\u6210\u635f\u4f24\uff0c\u751a\u81f3\u4f1a\u5f71\u54cd\u7ec6\u80de\u819cPS\u4e0eAnnexin V\u7684\u7ed3\u5408\u3002\u6d88\u5316\u65f6\u5c06\u80f0\u9176\u94fa\u6ee1\u5b54\u677f\u5e95\u540e\uff0c\u8f7b\u6447\u4f7f\u80f0\u9176\u4e0e\u7ec6\u80de\u5145\u5206\u63a5\u89e6\uff0c\u4e4b\u540e\u5012\u6389\u5927\u90e8\u5206\u80f0\u9176\uff0c\u5229\u7528\u6b8b\u7559\u7684\u5c11\u91cf\u80f0\u9176\u518d\u6d88\u5316\u4e00\u6bb5\u65f6\u95f4\uff0c\u5f85\u7ec6\u80de\u95f4\u7a7a\u9699\u589e\u5927\uff0c\u74f6\u5e95\u5448\u82b1\u6591\u72b6\u5373\u53ef\u7ec8\u6b62\u3002\u6d88\u5316\u6db2\u4e2d\u5c3d\u91cf\u4e0d\u7528EDTA\uff0cEDTA\u4f1a\u5f71\u54cdAnnexin V\u4e0ePS\u7684\u7ed3\u5408\u3002<\/span><\/p>\n

3\uff09 \u5b9e\u9a8c\u4e2d\u82e5\u9700\u56fa\u5b9a\u7ec6\u80de\uff0c\u6bd4\u5982\u51cb\u4ea1\u68c0\u6d4b\u540c\u65f6\u8fd8\u9700\u8981\u68c0\u6d4b\u7ec6\u80de\u5468\u671f\uff0c\u5efa\u8bae\u9009\u7528Annexin V-FITC\uff0c\u4e0d\u8981\u4f7f\u7528Annexin V-EGFP\uff0c\u56e0\u56fa\u5b9a\u8fc7\u7a0b\u4e2dEGFP\u4f1a\u53d8\u6027\u5bfc\u81f4\u65e0\u8367\u5149\u3002<\/span><\/p>\n

4\uff09 \u82e5\u6837\u54c1\u6765\u6e90\u4e8e\u8840\u6db2\uff0c\u52a1\u5fc5\u5148\u53bb\u9664\u8840\u6db2\u4e2d\u7684\u8840\u5c0f\u677f\u3002\u56e0\u4e3a\u8840\u5c0f\u677f\u542b\u6709PS\uff0c\u4f1a\u4e0eAnnexin V\u7ed3\u5408\u4ea7\u751f\u5047\u9633\u6027\u3002\u53ef\u4f7f\u7528\u542bEDTA\u7684\u7f13\u51b2\u6db2\u5e76\u4f4e\u901f\u79bb\u5fc3\u53bb\u9664\u8840\u5c0f\u677f\u3002<\/span><\/p>\n

5\uff09 \u8367\u5149\u7269\u8d28\u6613\u53d1\u751f\u6dec\u706d\uff0c\u8bd5\u5242\u50a8\u5b58\u548c\u64cd\u4f5c\u8fc7\u7a0b\u4e2d\u5c3d\u91cf\u907f\u5149\u3002\u8367\u5149\u68c0\u6d4b\u65f6\u4e5f\u5c3d\u91cf\u7f29\u77ed\u89c2\u5bdf\u65f6\u95f4\u3002<\/span><\/p>\n

6\uff09 \u4e3a\u4e86\u60a8\u7684\u5b89\u5168\u548c\u5065\u5eb7\uff0c\u8bf7\u7a7f\u5b9e\u9a8c\u670d\u5e76\u6234\u4e00\u6b21\u6027\u624b\u5957\u64cd\u4f5c\u3002<\/span><\/p>\n

\u4f7f\u7528\u65b9\u6cd5<\/strong><\/span><\/p>\n

\u4e00\u3001 \u67d3\u8272\u6b65\u9aa4<\/span><\/p>\n

1.1 \u7ec6\u80de\u6837\u54c1\u7684\u51c6\u5907\uff1a<\/span><\/p>\n

a\uff09\u5bf9\u4e8e\u8d34\u58c1\u7ec6\u80de\uff1a\u5c0f\u5fc3\u6536\u96c6\u7ec6\u80de\u57f9\u517b\u6db2\u5230\u4e00\u5e72\u51c0\u79bb\u5fc3\u7ba1\u5185\u5907\u7528\u3002 \u7528\u4e0d\u542bEDTA\u7684\u80f0\u9176\u6d88\u5316\u7ec6\u80de\u81f3\u7ec6\u80de\u53ef\u4ee5\u88ab\u8f7b\u8f7b\u7528\u79fb\u6db2\u7ba1\u6216\u67aa\u5934\u5439\u6253\u4e0b\u6765\u65f6\uff0c\u52a0\u5165\u524d\u9762\u6536\u96c6\u7684\u7ec6\u80de\u57f9\u517b\u6db2\uff0c\u5439\u6253\u4e0b\u6240\u6709\u7684\u8d34\u58c1\u7ec6\u80de\uff0c\u5e76\u8f7b\u8f7b\u5439\u6563\u7ec6\u80de\u3002\u518d\u6b21\u6536\u96c6\u5230\u79bb\u5fc3\u7ba1\u5185\u30021000rpm\u79bb\u5fc35min\uff0c\u6c89\u6dc0\u7ec6\u80de\u3002\u5bf9\u4e8e\u7279\u5b9a\u7684\u7ec6\u80de\uff0c\u5982\u679c\u65e0\u6cd5\u5b8c\u5168\u79bb\u5fc3\u81f3\u7ba1\u5e95\uff0c\u53ef\u4ee5\u9002\u5f53\u5ef6\u957f\u79bb\u5fc3\u65f6\u95f4\u6216\u7a0d\u7a0d\u52a0\u5927\u79bb\u5fc3\u901f\u5ea6\u3002\u5c0f\u5fc3\u5438\u9664\u4e0a\u6e05\uff0c\u53ef\u4ee5\u6b8b\u7559\u7ea650\u00b5l\u5de6\u53f3\u7684\u57f9\u517b\u6db2\uff0c\u4ee5\u907f\u514d\u5438\u8d70\u7ec6\u80de\u3002\u52a0\u51651ml 4\u2103\u9884\u51b7\u7684PBS\uff0c\u91cd\u60ac\u7ec6\u80de\uff0c\u518d\u6b21\u79bb\u5fc3\u6c89\u6dc0\u7ec6\u80de\uff0c\u5c0f\u5fc3\u5438\u9664\u4e0a\u6e05\u3002<\/span><\/p>\n

b\uff09\u5bf9\u4e8e\u60ac\u6d6e\u7ec6\u80de\uff1a1000rpm\u79bb\u5fc35min\u6c89\u6dc0\u7ec6\u80de\u3002\u5bf9\u4e8e\u7279\u5b9a\u7684\u7ec6\u80de\uff0c\u5982\u679c\u65e0\u6cd5\u5b8c\u5168\u79bb\u5fc3\u81f3\u7ba1\u5e95\uff0c\u53ef\u4ee5\u9002\u5f53\u5ef6\u957f\u79bb\u5fc3\u65f6\u95f4\u6216\u7a0d\u7a0d\u52a0\u5927\u79bb\u5fc3\u901f\u5ea6\u3002\u5c0f\u5fc3\u5438\u9664\u4e0a\u6e05\uff0c\u53ef\u4ee5\u6b8b\u7559\u7ea650\u00b5l\u5de6\u53f3\u7684\u57f9\u517b\u6db2\uff0c\u4ee5\u907f\u514d\u5438\u8d70\u7ec6\u80de\u3002\u52a0\u51651ml 4\u2103\u9884\u51b7\u7684PBS\uff0c\u91cd\u60ac\u7ec6\u80de\uff0c\u518d\u6b21\u79bb\u5fc3\u6c89\u6dc0\u7ec6\u80de\uff0c\u5c0f\u5fc3\u5438\u9664\u4e0a\u6e05\u3002<\/span><\/p>\n

1.2 \u7528\u53bb\u79bb\u5b50\u6c34\u6309\u71671:3\u7a00\u91ca\u7ed3\u5408\u7f13\u51b2\u6db2\u52301\u00d7\uff0c\u6bd4\u5982\uff0c5ml\u7ed3\u5408\u7f13\u51b2\u6db2\uff084\u00d7\uff09\u52a0\u516515ml\u53bb\u79bb\u5b50\u6c34\u3002<\/span><\/p>\n

1.3 \u7528\u7ed3\u5408\u7f13\u51b2\u6db2\uff081\u00d7\uff09\u91cd\u65b0\u60ac\u6d6e\u7ec6\u80de\u5e76\u8c03\u6574\u6d53\u5ea6\u4e3a1-5\u00d7106\/ml\u3002<\/span><\/p>\n

1.4 \u53d6100\u00b5l\u7684\u7ec6\u80de\u60ac\u6db2\u52305ml\u6d41\u5f0f\u7ba1\u4e2d\uff0c\u52a0\u51655\u00b5l Annexin V-FITC\u6df7\u5300\uff0c\u5ba4\u6e29\u907f\u5149\u5b75\u80b25min\u3002<\/span><\/p>\n

1.5 \u518d\u52a0\u516510\u00b5l\u7898\u5316\u4e19\u5576\u6eb6\u6db2\uff0c\u5e76\u52a0400\u00b5l\u7ed3\u5408\u7f13\u51b2\u6db2\uff081\u00d7\uff09\uff0c\u6df7\u5300\uff0c\u5c3d\u5feb\uff08\uff1c1h\uff09\u7528\u6d41\u5f0f\u7ec6\u80de\u4eea\u6216\u8367\u5149\u663e\u5fae\u955c\u5b8c\u6210\u68c0\u6d4b\u3002<\/span><\/p>\n

\u3010\u6ce8\u610f\u3011\uff1a\u65b9\u4fbf\u8d77\u89c1\uff0c\u6b65\u9aa41.4\u5185\u53ef\u540c\u65f6\u52a0\u51655\u00b5l Annexin V-FITC\u548c10\u00b5l\u7898\u5316\u4e19\u5576\u6eb6\u6db2\u6df7\u5300\uff0c\u5ba4\u6e29\u907f\u5149\u5b75\u80b25min\u3002\u7136\u540e\u52a0\u5165400\u00b5l\u7ed3\u5408\u7f13\u51b2\u6db2\uff081\u00d7\uff09\u6df7\u5300\uff0c\u4f46\u9700\u5c3d\u5feb\uff08\uff1c1h\uff09\u7528\u6d41\u5f0f\u7ec6\u80de\u4eea\u6216\u8367\u5149\u663e\u5fae\u955c\u5b8c\u6210\u68c0\u6d4b\u3002\u4f46\u4e3a\u4e86\u51cf\u5c11\u5b9e\u9a8c\u8bef\u5dee\uff0c\u5efa\u8bae\u6309\u71671.4-1.5\u7684\u6b65\u9aa4\u6765\u64cd\u4f5c\u3002\u56e0PI\u53d7\u65f6\u95f4\u5f71\u54cd\u6bd4\u8f83\u5927\uff0c\u7ec6\u80de\u6807\u8bb0\u4e0aPI\u540e\u6bd2\u6027\u4f1a\u589e\u52a0\u3002\u968f\u7740\u65f6\u95f4\u5ef6\u957f\u4f1a\u5bfc\u81f4PI\u67d3\u8272\u589e\u52a0\uff0c\u5c24\u5176\u662f\u68c0\u6d4b\u65e9\u671f\u51cb\u4ea1\u65f6\uff0c\u8bef\u5dee\u4f1a\u660e\u663e\u52a0\u5927\u3002\u5efa\u8baePI\u52a0\u5165\u540e\u7acb\u5373\u5f00\u59cb\u68c0\u6d4b\u3002<\/span><\/p>\n

\u4e8c\u3001 \u67d3\u8272\u68c0\u6d4b<\/strong><\/span><\/p>\n

2.1\u6d41\u5f0f\u7ec6\u80de\u4eea\u5206\u6790<\/span><\/p>\n

1\uff09\u8bf7\u6839\u636e\u4ee5\u4e0b\u8bbe\u8ba1\u5b9e\u9a8c\uff1a<\/span><\/p>\n

\u7a7a\u767d\u7ba1\uff1a\u9634\u6027\u5bf9\u7167\u7ec4\u7ec6\u80de\uff0c\u4e0d\u52a0Annexin V-FITC\u548c\u7898\u5316\u4e19\u5576\u6eb6\u6db2\u3002\u76ee\u7684\u7528\u6765\u8c03\u8282\u7535\u538b\u3002<\/span><\/p>\n

\u5355\u67d3\u7ba1\uff1a\u9633\u6027\u5bf9\u7167\u7ec4\u7ec6\u80de\uff0c\u53ea\u52a0Annexin V-FITC\u3002\u76ee\u7684\u7528\u6765\u8c03\u8282\u8865\u507f\u3002<\/span><\/p>\n

\u68c0\u6d4b\u7ba1\uff1a\u5904\u7406\u7ec6\u80de\u7ec4\uff0c\u52a0Annexin V-FITC\u548c\u7898\u5316\u4e19\u5576\u6eb6\u6db2\u3002\u7528\u7a7a\u767d\u7ba1\u548c\u5355\u67d3\u7ba1\u8c03\u8282\u597d\u7535\u538b\u8865\u507f\u540e\uff0c\u83b7\u5f97\u6240\u9700\u7684\u6d41\u5f0f\u6570\u636e\u3002<\/span><\/p>\n

2\uff09\u5bf9\u4e8eAnnexin V-FITC\uff08Ex=488nm\uff0cEm=525nm\uff09\uff0c\u7528\u6d41\u5f0f\u7ec6\u80de\u4eea\u7684FITC\u4fe1\u53f7\u68c0\u6d4b\u5668\uff08\u901a\u5e38F1\u901a\u9053\uff09\uff1b\u5bf9\u4e8ePI-DNA\u590d\u5408\u7269\uff08Ex=535nm\uff0cEm=615nm\uff09\uff0c\u7528\u6d41\u5f0f\u7ec6\u80de\u4eea\u7684PE\u53d1\u5c04\u4fe1\u53f7\u68c0\u6d4b\u5668\uff08\u901a\u5e38F2\u901a\u9053\uff09\u3002<\/span><\/p>\n

3\uff09\u7528CellQuest\u7b49\u8f6f\u4ef6\u8fdb\u884c\u5206\u6790\uff0c\u7ed8\u5236\u53cc\u8272\u6563\u70b9\u56fe\uff08two-color dot plot\uff09\uff0cFITC\u4e3a\u6a2a\u5750\u6807\uff0cPI\u4e3a\u7eb5\u5750\u6807\u3002\u6bcf\u4e2a\u6837\u91c7\u96c610,000 events\u3002\u5178\u578b\u5b9e\u9a8c\u7ed3\u679c\u4e2d\uff0c\u7ec6\u80de\u53ef\u5206\u6210\u4e09\u4e2a\u4e9a\u7fa4\uff0c\u6d3b\u7ec6\u80de\u4ec5\u6709\u5f88\u4f4e\u5f3a\u5ea6\u7684\u80cc\u666f\u8367\u5149\uff0c\u51cb\u4ea1\u65e9\u671f\u7ec6\u80de\u4ec5\u6709\u8f83\u5f3a\u7684\u7eff\u8272\u8367\u5149\uff0c\u51cb\u4ea1\u665a\u671f\u7ec6\u80de\u6216\u574f\u6b7b\u7ec6\u80de\u6709\u7eff\u8272\u548c\u7ea2\u8272\u7684\u53cc\u91cd\u8367\u5149\u67d3\u8272\u3002<\/span><\/p>\n

2.2\u8367\u5149\u663e\u5fae\u955c\u5206\u6790<\/strong><\/span><\/p>\n

1)\u6ef4\u4e00\u6ef4\u7528Annexin V-FITC\/PI\u53cc\u67d3\u7684\u7ec6\u80de\u60ac\u6db2\u4e8e\u8f7d\u73bb\u7247\u4e0a\uff0c\u5e76\u7528\u76d6\u73bb\u7247\u76d6\u4e0a\u7ec6\u80de\u3002\u3010\u6ce8\u610f\u3011\uff1a\u5bf9\u4e8e\u8d34\u58c1\u7ec6\u80de\uff0c\u53ef\u76f4\u63a5\u7528\u76d6\u73bb\u7247\u57f9\u517b\u7ec6\u80de\u5e76\u8bf1\u5bfc\u7ec6\u80de\u51cb\u4ea1\u3002\u63a2\u9488\u5b75\u80b2\u7ed3\u675f\u540e\uff0c\u53ef\u5c06\u76d6\u73bb\u7247\u5012\u7f6e\u5728\u8f7d\u73bb\u7247\u4e0a\u5e76\u8fdb\u884c\u89c2\u5bdf\u3002\u9700\u8981\u5728\u67d3\u8272\u7ed3\u675f\u540e\u5c3d\u5feb\uff08\uff1c1h\uff09\u8fdb\u884c\u89c2\u5bdf\u3002<\/span><\/p>\n

2)\u4e8e\u8367\u5149\u663e\u5fae\u955c\u4e0b\u7528\u53cc\u8272\u6ee4\u5149\u7247\uff08FITC\/\u7f57\u4e39\u660e\uff09\u8fdb\u884c\u89c2\u5bdf\u3002\u65e9\u671f\u51cb\u4ea1\u7ec6\u80de\uff0c\u4ec5\u7ed3\u5408Annexin V-FITC\u7684\u7ec6\u80de\u8d28\u819c\u5448\u73b0\u7eff\u8272\u3002\u800c\u4e27\u5931\u819c\u5b8c\u6574\u7684\u7ec6\u80de\u5728\u7ec6\u80de\u6838\u4e0a\u88abPI\u67d3\u8272\u5448\u73b0\u7ea2\u8272\uff0c\u4e14\u5728\u7ec6\u80de\u8868\u9762\uff08\u8d28\u819c\uff09\u4e0a\u56e0\u88abAnnexin V-FITC\u67d3\u8272\u5448\u7eff\u8272\u5149\u73af\u3002<\/span><\/p>\n

\u5e38\u89c1\u95ee\u9898<\/strong><\/span><\/p>\n

1\uff09 Annexin V\/PI\u51cb\u4ea1\u8bd5\u5242\u76d2\u80fd\u591f\u7528\u4e8e\u68c0\u6d4b\u4eba\u6216\u5176\u4ed6\u52a8\u7269\u7684\u7ec6\u80de\u51cb\u4ea1\u60c5\u51b5\uff1f<\/span><\/p>\n

\u56de\u590d\uff1a\u53ef\u4ee5\uff0c\u56e0Annexin V\u7279\u5f02\u6027\u7ed3\u5408PS\uff0c\u800cPS\u5728\u4e0d\u540c\u79cd\u5c5e\u95f4\u65e0\u5dee\u5f02\u3002<\/span><\/p>\n

2\uff09\u8d34\u58c1\u7ec6\u80de\u505a\u51cb\u4ea1\u7528\u80f0\u9176\u6d88\u5316\u5bf9\u7ec6\u80de\u819c\u6709\u635f\u4f24\uff1f<\/span><\/p>\n

\u56de\u590d\uff1a\u4f4e\u6d53\u5ea6\u80f0\u9176\u6d88\u5316\uff0c\u8f7b\u67d4\u5439\u6253\u8d34\u58c1\u7ec6\u80de2\uff5e3\u6b21\uff0c4\u2103\uff0c1000rpm\u79bb\u5fc35min\uff0c \u5904\u7406\u5f97\u5f53\u7684\u8bdd\uff0c \u80f0\u9176\u9020\u6210\u635f\u4f24\u53ef\u4ee5\u63a7\u5236\u5728 5%\u4ee5\u5185\uff0c\u5728\u6709\u5bf9\u7167\u7ec4\u7684\u60c5\u51b5\u4e0b\u5bf9\u5b9e\u9a8c\u7ed3\u679c\u4e0d\u4f1a\u9020\u6210\u660e\u663e\u5f71\u54cd\u3002<\/span><\/p>\n

3\uff09\u8d34\u58c1\u7ec6\u80de\u53ef\u5148\u67d3PI\u7136\u540e\u518d\u6d88\u5316\u5417\uff1f\u8fd9\u6837\u662f\u5426\u53ef\u51cf\u5c11\u7531\u4e8e\u6d88\u5316\u6db2\u9020\u6210\u7684\u7ec6\u80de\u819c\u635f\u4f24\u800c\u67d3\u4e0aPI\u7684\u8bef\u5dee\uff1f<\/span><\/p>\n

\u56de\u590d\uff1a\u5148\u52a0 PI\u4e0d\u4ec5\u67d3\u8272\u662f\u5426\u6bcf\u7ec4\u90fd\u5747\u5300\u5145\u5206\u5f88\u96be\u5224\u65ad\uff0c \u800c\u4e14PI\u672c\u8eab\u5bf9\u7ec6\u80de\u4e5f\u662f\u6709\u6bd2\u6027\u7684\uff0c \u5bf9\u5b9e\u9a8c\u7ed3\u679c\u5f71\u54cd\u4f1a\u6bd4\u80f0\u9176\u5927\uff0c\u4e0d\u5efa\u8bae\u8fd9\u6837\u505a\u3002<\/span><\/p>\n

4\uff09\u4e3a\u4ec0\u4e48\u53ea\u80fd\u7528\u4e0d\u542b EDTA\u7684\u80f0\u9176\u6d88\u5316\u8d34\u58c1\u7ec6\u80de\uff0c\u7528\u542b EDTA\u7684\u80f0\u9176\u6d88\u5316\u7ec6\u80de\u5bf9\u7ed3\u679c\u6709\u4ec0\u4e48\u5f71\u54cd\uff1f<\/span><\/p>\n

\u56de\u590d\uff1a\u56e0Annexin V\u662f\u9499\u79bb\u5b50\u4f9d\u8d56\u6027\u7684\u78f7\u8102\u7ed3\u5408\u86cb\u767d\uff0cEDTA\u662f\u4e00\u79cd\u91d1\u5c5e\u87af\u5408\u5242\u3002\u56e0\u6b64EDTA\u7684\u52a0\u5165\u4f1a\u5f71\u54cdAnnexin V\u6d3b\u6027\uff0c\u8fdb\u800c\u5f71\u54cd\u7ed3\u679c\u3002<\/span><\/p>\n

\u76f8\u5173\u4ea7\u54c1<\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n
\u8d27\u53f7<\/span><\/td>\n\u540d\u79f0<\/span><\/td>\n\u89c4\u683c<\/span><\/td>\n<\/tr>\n
MX3210-20T<\/span><\/td>\nAnnexin V-FITC\/PI Apoptosis Detection Kit\u7ec6\u80de\u51cb\u4ea1\u68c0\u6d4b\u8bd5\u5242\u76d2<\/span><\/td>\n20T<\/span><\/td>\n<\/tr>\n
MX3211-20T<\/span><\/td>\nAnnexin V-Alexa Fluor 488\/PI Apoptosis Detection Kit\u7ec6\u80de\u51cb\u4ea1\u68c0\u6d4b\u8bd5\u5242\u76d2<\/span><\/td>\n20T<\/span><\/td>\n<\/tr>\n
MX3212-20T<\/span><\/td>\nAnnexin V-EGFP\/PI Apoptosis Detection Kit\u7ec6\u80de\u51cb\u4ea1\u68c0\u6d4b\u8bd5\u5242\u76d2<\/span><\/td>\n20T<\/span><\/td>\n<\/tr>\n
MX3213-20T<\/span><\/td>\nAnnexin V-Alexa Fluor 647\/PI Apoptosis Detection Kit\u7ec6\u80de\u51cb\u4ea1\u68c0\u6d4b\u8bd5\u5242\u76d2<\/span><\/td>\n20T<\/span><\/td>\n<\/tr>\n
MX3214-30T<\/span><\/td>\nAnnexin V-APC\/7-AAD Apoptosis Detection Kit \u7ec6\u80de\u51cb\u4ea1\u68c0\u6d4b\u8bd5\u5242\u76d2<\/span><\/td>\n30T<\/span><\/td>\n<\/tr>\n
MX3215-20T<\/span><\/td>\nAnnexin V-PE\/7-AAD Apoptosis Detection Kit\u7ec6\u80de\u51cb\u4ea1\u68c0\u6d4b\u8bd5\u5242\u76d2<\/span><\/td>\n20T<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u2014 \u2014Written\/Edited by V.<\/strong> Shallan\u3010\u7248\u6743\u5f52\u96c6\u5947\u751f\u7269\/MKBio\u61cb\u5eb7\u6240\u6709\u3011<\/span><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u66f4\u591a\u4ea7\u54c1\u4fe1\u606f\u8bf7\u54a8\u8be2\u5ba2\u670d\uff01<\/p>\n","protected":false},"featured_media":26239,"template":"","meta":[],"product_cat":[99],"product_tag":[105],"_links":{"self":[{"href":"\/\/www.5080666.com\/wp-json\/wp\/v2\/product\/3666"}],"collection":[{"href":"\/\/www.5080666.com\/wp-json\/wp\/v2\/product"}],"about":[{"href":"\/\/www.5080666.com\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"\/\/www.5080666.com\/wp-json\/wp\/v2\/media\/26239"}],"wp:attachment":[{"href":"\/\/www.5080666.com\/wp-json\/wp\/v2\/media?parent=3666"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"\/\/www.5080666.com\/wp-json\/wp\/v2\/product_cat?post=3666"},{"taxonomy":"product_tag","embeddable":true,"href":"\/\/www.5080666.com\/wp-json\/wp\/v2\/product_tag?post=3666"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}