{"id":3247,"date":"2019-07-31T16:44:08","date_gmt":"2019-07-31T08:44:08","guid":{"rendered":"\/\/www.5080666.com\/product\/benzonase-nuclease-%e5%85%a8%e8%83%bd%e6%a0%b8%e9%85%b8%e9%85%b6\/"},"modified":"2022-03-04T10:14:27","modified_gmt":"2022-03-04T02:14:27","slug":"benzonase-nuclease-%e5%85%a8%e8%83%bd%e6%a0%b8%e9%85%b8%e9%85%b6","status":"publish","type":"product","link":"\/\/www.5080666.com\/product\/benzonase-nuclease-%e5%85%a8%e8%83%bd%e6%a0%b8%e9%85%b8%e9%85%b6\/","title":{"rendered":"Benzonase Nuclease \u5168\u80fd\u6838\u9178\u9176"},"content":{"rendered":"

Benzonase Nuclease \u5168\u80fd\u6838\u9178\u9176\/\u5e7f\u8c31\u6838\u9178\u9176<\/strong><\/span><\/p>\n

\uff08\u9175\u6bcd\u91cd\u7ec4\u8868\u8fbe\uff0c\u65e0\u7ec6\u83cc\u5185\u6bd2\u7d20\u6b8b\u7559\uff0c\u65e0\u86cb\u767d\u9176\u6c61\u67d3\uff09<\/span><\/p>\n

\u4ea7\u54c1\u5173\u952e\u8bcd\uff1a<\/span><\/strong><\/span><\/p>\n

Benzonase Nuclease \u5168\u80fd\u6838\u9178\u9176\uff08\u5e7f\u8c31\u6838\u9178\u9176\uff09\uff1b\u7c98\u8d28\u6c99\u96f7\u6c0f\u83cc\uff08Serratia Marcescen\uff09\uff1b\u975e\u7279\u5f02\u6027\u6838\u9178\u5185\u5207\u9176\uff1bRIPA\u88c2\u89e3\u6db2\uff1bDNA\/RNA\u964d\u89e3\uff1bCAS\uff1a9025-65-4\uff1b<\/span><\/p>\n

\u8ba2\u8d2d\u4fe1\u606f\uff1a<\/span><\/strong>\u66f4\u5927\u5305\u88c5\u6216\u4ea7\u54c1\u6280\u672f\u95ee\u9898\uff0c\u8bf7\u6765\u7535\/\u5728\u7ebf\u54a8\u8be2\uff0c\u7535\u8bdd\uff1a021-54736159\u3002QQ\uff1a2971634497\u3002<\/span><\/p>\n

\n \n \n \n \n \n
\u8d27\u53f7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/td>\n \u4ea7\u54c1\u540d\u79f0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/span><\/td>\n \u89c4\u683c\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/td>\n CAS NO.\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/td>\n \u4ef7\u683c\uff08\u5143\uff09 \u00a0<\/span><\/td>\n \u8d27\u671f\u00a0\u00a0<\/span>\u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/td>\n<\/tr>\n
MP1509-25KU<\/span><\/td>\n Benzonase Nuclease \u5168\u80fd\u6838\u9178\u9176<\/span><\/td>\n 25KU<\/span><\/td>\n 9025-65-4<\/span><\/td>\n 400<\/span><\/td>\n \u73b0\u8d27<\/span><\/td>\n<\/tr>\n
MP1509-50KU<\/span><\/td>\n Benzonase Nuclease \u5168\u80fd\u6838\u9178\u9176<\/span><\/td>\n 50KU<\/span><\/td>\n 9025-65-4<\/span><\/td>\n 720<\/span><\/td>\n \u73b0\u8d27<\/span><\/td>\n<\/tr>\n
MP1509-100KU<\/span><\/td>\n Benzonase Nuclease \u5168\u80fd\u6838\u9178\u9176<\/span><\/td>\n 100KU<\/span><\/td>\n 9025-65-4<\/span><\/td>\n 1380<\/span><\/td>\n \u73b0\u8d27<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u6211\u53f8\u63d0\u4f9b\u7684Benzonase\uff0c\u6bd4Merck\u3001Sigma\u540c\u7b49\u7c7b\u578b\u7684\u4ea7\u54c1\u6027\u4ef7\u6bd4\u9ad8\u5f88\u591a\uff0c\u4e14\u57fa\u672c\u6709\u73b0\u8d27<\/span><\/strong>\uff0c\u89c1\u4e0b\uff1a<\/span><\/p>\n

\n\n\n\n\n\n\n
\u54c1\u724c\/\u540d\u79f0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0 <\/span><\/td>\n \u8d27\u53f7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/td>\n \u89c4\u683c\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/td>\n \u6d53\u5ea6\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/td>\n \u4ef7\u683c\uff08\u5143\uff09\u00a0 <\/span><\/td>\n<\/tr>\n
Merck\uff08Benzonase Nuclease,\u7eaf\u5ea6>90%\uff0cE. Coli\uff09<\/span><\/td>\n 70746-3<\/span><\/td>\n 10KU<\/span><\/td>\n 25 U\/\u03bcL<\/span><\/td>\n 1670<\/span><\/td>\n<\/tr>\n
Merck\uff08Benzonase Nuclease,\u7eaf\u5ea6>90%\uff0cE. Coli\uff09<\/span><\/td>\n 71205-3<\/span><\/td>\n 25KU<\/span><\/td>\n 250 U\/\u03bcL<\/span><\/td>\n 2530<\/span><\/td>\n<\/tr>\n
Sigma\uff08Benzonase Nuclease,\u7eaf\u5ea6>90%\uff0cE. Coli\uff09<\/span><\/td>\n E1014<\/span><\/td>\n 5KU<\/span><\/td>\n \u2265250 U\/\u03bcL<\/span><\/td>\n 643<\/span><\/td>\n<\/tr>\n
Sigma\uff08Benzonase Nuclease,\u7eaf\u5ea6>90%\uff0cE. Coli\uff09<\/span><\/td>\n E1014<\/span><\/td>\n 25KU<\/span><\/td>\n \u2265250 U\/\u03bcL<\/span><\/td>\n 2254<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u3010\u6ce8\u3011\uff1aBenzonase is a registered trademark of Merck KGaA, Darmstadt, Germany<\/span><\/span><\/p>\n

\u57fa\u672c\u63cf\u8ff0\uff1a<\/strong><\/span><\/p>\n

Benzonase\uff0c\u4e2d\u6587\u540d\u5168\u80fd\u6838\u9178\u9176\uff0c\u6216\u5e7f\u8c31\u6838\u9178\u9176<\/span>\uff0c\u4e00\u79cd\u6e90\u4e8e\u7c98\u8d28\u6c99\u96f7\u6c0f\u83cc\uff08Serratia Marcescen\uff0c\u53c8\u79f0\u4e3a\u7075\u6746\u83cc\uff09\u7684\u975e\u7279\u5f02\u6027\u6838\u9178\u5185\u5207\u9176\uff0c\u7531\u4e24\u4e2a\u5b8c\u5168\u4e00\u6837\u7684\u4e9a\u57fa\u6784\u6210\uff0c\u6bcf\u4e2a\u4e9a\u57fa\u5927\u7ea630kDa\uff0c\u542b\u6709245\u4e2a\u6c28\u57fa\u9178\u548c\u4e24\u4e2a\u5fc5\u9700\u7684\u4e8c\u786b\u952e\u3002\u53ef\u4ee5\u5207\u5272\u548c\u964d\u89e3\u5404\u79cd\u5f62\u5f0f\u7684DNA\u548cRNA\uff08\u5355\u94fe\uff0c\u53cc\u94fe\uff0c\u7ebf\u6027\uff0c\u73af\u72b6\uff0c\u6216\u8d85\u87ba\u65cb\u5f62\u5f0f\uff09\uff0c\u751f\u6210\u542b\u67095\u2019-\u78f7\u9178\u672b\u7aef\u76843-5\u4e2a\u5be1\u6838\u82f7\u9178\u6b8b\u57fa\u7247\u6bb5\uff0c\u5e76\u4e14\u5728\u5f88\u591a\u79cd\u64cd\u4f5c\u6761\u4ef6\u4e0b\u6709\u6548\u3002\u5bf9\u6838\u9178\u78b1\u57fa\u5e8f\u5217\u65e0\u7279\u5f02\u6027\u8981\u6c42\uff0c\u53ef\u5728\u94fe\u5185\u4efb\u610f\u6838\u82f7\u9178\u95f4\u8fdb\u884c\u5207\u5272\uff0c\u5e7f\u6cdb\u7528\u4e8e\u53bb\u9664\u751f\u7269\u5236\u54c1\u4e2d\u7684\u6838\u9178\u3002<\/span><\/p>\n

\u672c\u54c1\u7ecf\u57fa\u56e0\u5de5\u7a0b\u6539\u9020\u7684\u771f\u6838\u751f\u7269\u9175\u6bcd\u83cc\u8868\u8fbe\u7eaf\u5316\u6240\u5f97\uff0c\u4e0d\u542b\u539f\u6838\u751f\u7269\u8868\u8fbe\u7cfb\u7edf\u643a\u5e26\u7684\u7ec6\u83cc\u5185\u6bd2\u7d20\u3002\u4e0d\u4ec5\u53ef\u4f5c\u4e3a\u57f9\u517b\u7ec6\u80de\u4e0a\u6e05\u548c\u7ec6\u80de\u88c2\u89e3\u6db2\u53bb\u7c98\u5ea6\u7684\u9996\u9009\u9176\u5236\u5242\uff0c\u53bb\u9664\u6838\u9178\u5e72\u6270\u63d0\u9ad8\u540e\u7eed\u86cb\u767d\u7eaf\u5316\u6216\u529f\u80fd\u7814\u7a76\uff1b\u800c\u4e14\u53ef\u5e94\u7528\u5728\u75ab\u82d7\u5de5\u4e1a\u3001\u86cb\u767d\u548c\u591a\u7cd6\u7c7b\u5236\u836f\u5de5\u4e1a\uff0c\u53bb\u9664\u5bbf\u4e3b\u6838\u9178\u6b8b\u7559\uff0c\u4f7f\u5f97\u75ab\u82d7\u548c\u86cb\u767d\u7c7b\u6838\u9178\u6c61\u67d3\u663e\u8457\u964d\u4f4e\u81f3\u76ae\u514b\u7ea7\u522b\uff0c\u63d0\u4f9b\u751f\u7269\u529f\u6548\u3002<\/span><\/p>\n

\u672c\u54c1\u4ee5\u65e0\u83cc\u6db2\u4f53\u9176<\/span>\u7684\u5f62\u5f0f\u63d0\u4f9b\uff0c\u50a8\u5b58\u7f13\u51b2\u6db2\u4e3a20mM Tris-Cl pH8.0\uff0c2mM MgCl2\uff0c2mM NaCl\uff0c50%\u7518\u6cb9\u3002<\/span><\/p>\n

\u4ea7\u54c1\u7279\u6027\uff1a<\/strong><\/span><\/p>\n

1\uff09CAS NO\uff1a9025-65-4<\/span><\/p>\n

2\uff09\u540c\u4e49\u540d\uff1aBenzNuclease; Universal Nuclease; Benzonase endonuclease<\/span><\/p>\n

3\uff09\u5206\u5b50\u91cf\uff1a27.9kDa<\/span><\/p>\n

4\uff09\u7eaf\u5ea6\uff1a\u2265 90%\uff08SDS-PAGE\uff09<\/span><\/p>\n

5\uff09\u9176\u6d3b\u6027\u503c\uff1a\u2265250 U\/\u03bcL<\/span><\/p>\n

6\uff09\u6bd4\u6d3b\u6027\u503c\uff1a\u22651.0\u00d7106U\/mg\u86cb\u767d<\/span><\/p>\n

7\uff09\u86cb\u767d\u9176\uff1a\u672a\u68c0\u51fa<\/span><\/p>\n

8\uff09\u5185\u6bd2\u7d20\uff1a\u672a\u68c0\u51fa\uff08\u9c8e\u8bd5\u5242\u6cd5\uff09<\/span><\/p>\n

9\uff09\u6700\u9002pH\uff1a8.0\uff08\u5de5\u4f5c\u8303\u56f4pH<\/a>6-10\uff09<\/span><\/p>\n

10\uff09\u6700\u9002\u6e29\u5ea6\uff1a37\u2103\uff08\u5de5\u4f5c\u8303\u56f40-42\u2103\uff09<\/span><\/p>\n

11\uff09\u8f85\u52a9\u56e0\u5b50\uff1a1\uff5e10 mM Mg2+<\/span><\/p>\n

12\uff09\u7a00\u91ca\u7f13\u51b2\u6db2\uff1a20mM Tris-Cl pH8.0, 2mM MgCl2, 2mM NaCl<\/span><\/p>\n

13\uff09\u6d3b\u6027\u5355\u4f4d\u5b9a\u4e49\uff1a\u572837\u2103\uff0cpH8.0\u53cd\u5e94\u6761\u4ef6\uff0c2.625ml\u53cd\u5e94\u4f53\u7cfb\u4e2d\uff0c30min\u5185\u4f7f\u25b3A260\u5438\u6536\u503c\u53d8\u53161.0\uff08\u76f8\u5f53\u4e8e\u5b8c\u5168\u6d88\u531637\u03bcg\u9c91\u9c7c\u7cbeDNA\u6210\u4e3a\u5be1\u6838\u82f7\u9178\uff09\u6240\u7528\u7684\u9176\u91cf\u5b9a\u4e49\u4e3a\u4e00\u4e2a\u6d3b\u6027\u5355\u4f4d\uff08U\uff09\u3002<\/span><\/p>\n

\u4fdd\u5b58\u4e0e\u8fd0\u8f93\u65b9\u6cd5<\/strong><\/span><\/p>\n

\u4fdd\u5b58\uff1a-20\u2103\u51bb\u5b58\uff0c1\u5e74\u7a33\u5b9a\u3002\u3010\u6ce8\u3011\uff1a\u4e0d\u8981\u7f6e\u4e8e-70\u2103\uff0c\u56e0\u51b7\u51bb\u7ed3\u51b0\u4f1a\u9020\u6210\u9176\u6d3b\u6027\u7684\u4e27\u5931\u3002<\/span><\/p>\n

\u8fd0\u8f93\uff1a\u51b0\u888b\u8fd0\u8f93\u3002<\/span><\/p>\n

\u76f8\u5173\u4ea7\u54c1\uff1a<\/span><\/strong>\u66f4\u5927\u5305\u88c5\u6216\u4ea7\u54c1\u6280\u672f\u95ee\u9898\uff0c\u8bf7\u6765\u7535\/\u5728\u7ebf\u54a8\u8be2\uff0c\u7535\u8bdd\uff1a021-54736159\u3002QQ\uff1a2971634497\u3002<\/span><\/p>\n

\n\n\n\n\n\n\n\n\n
\u8d27\u53f7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/td>\n \u4ea7\u54c1\u540d\u79f0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/span><\/td>\n \u89c4\u683c\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/td>\n \u4ef7\u683c\uff08\u5143\uff09\u00a0\u00a0<\/span><\/td>\n \u8d27\u671f\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/span><\/td>\n<\/tr>\n
MP1501-100ML<\/span><\/td>\n RIPA Lysis Buffer (Strong) RIPA\u88c2\u89e3\u6db2\uff08\u5f3a\uff09<\/span><\/td>\n 100ml<\/span><\/td>\n 225<\/span><\/td>\n \u73b0\u8d27<\/span><\/td>\n<\/tr>\n
MP1502-100ML<\/span><\/td>\n RIPA Lysis Buffer (Medium) RIPA\u88c2\u89e3\u6db2\uff08\u4e2d\uff09<\/span><\/td>\n 100ml<\/span><\/td>\n 225<\/span><\/td>\n \u73b0\u8d27<\/span><\/td>\n<\/tr>\n
MP1503-100ML<\/span><\/td>\n RIPA Lysis Buffer (Weak) RIPA\u88c2\u89e3\u6db2\uff08\u5f31\uff09<\/span><\/td>\n 100ml<\/span><\/td>\n 225<\/span><\/td>\n \u73b0\u8d27<\/span><\/td>\n<\/tr>\n
MP1504-100ML<\/span><\/td>\n Lysis Buffer for WB\/IP Assays<\/span><\/p>\n

\u514d\u75ab\u5370\u8ff9\u53ca\u514d\u75ab\u6c89\u6dc0\u7528\uff08WB\/IP\uff09\u88c2\u89e3\u6db2<\/span><\/td>\n

 100ml<\/span><\/td>\n 225<\/span><\/td>\n \u73b0\u8d27<\/span><\/td>\n<\/tr>\n
MP1505-100ML<\/span><\/td>\n NP-40 Lysis Buffer NP-40\u88c2\u89e3\u6db2<\/span><\/td>\n 100ml<\/span><\/td>\n 225<\/span><\/td>\n \u73b0\u8d27<\/span><\/td>\n<\/tr>\n
MP1506-100ML<\/span><\/td>\n SDS Lysis Buffer SDS\u88c2\u89e3\u6db2<\/span><\/td>\n 100ml<\/span><\/td>\n 225<\/span><\/td>\n \u73b0\u8d27<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u6ce8\u610f\u4e8b\u9879<\/strong><\/span><\/p>\n

\u4e3a\u4e86\u60a8\u7684\u5b89\u5168\u548c\u5065\u5eb7\uff0c\u8bf7\u7a7f\u5b9e\u9a8c\u670d\u5e76\u6234\u4e00\u6b21\u6027\u624b\u5957\u64cd\u4f5c\u3002<\/span><\/p>\n

\u672c\u4ea7\u54c1\u4ec5\u80fd\u7528\u4f5c\u79d1\u7814\u7528\u9014\uff0c\u4e0d\u53ef\u4ed6\u7528\u3002<\/span><\/p>\n

\u00a0\u2014 \u2014 Written\/Edited by V. Shallan\u3010\u7248\u6743\u5f52MKBio\u61cb\u5eb7\u6240\u6709\u3011<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

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