{"id":299,"date":"2019-07-24T10:28:16","date_gmt":"2019-07-24T02:28:16","guid":{"rendered":"\/\/www.5080666.com\/product\/hygromycin-b-%e6%bd%ae%e9%9c%89%e7%b4%a0b%ef%bc%88%e5%86%bb%e5%b9%b2%e7%b2%89%ef%bc%89-3\/"},"modified":"2021-11-22T17:54:56","modified_gmt":"2021-11-22T09:54:56","slug":"hygromycin-b-%e6%bd%ae%e9%9c%89%e7%b4%a0b%ef%bc%88%e5%86%bb%e5%b9%b2%e7%b2%89%ef%bc%89-3","status":"publish","type":"product","link":"\/\/www.5080666.com\/product\/hygromycin-b-%e6%bd%ae%e9%9c%89%e7%b4%a0b%ef%bc%88%e5%86%bb%e5%b9%b2%e7%b2%89%ef%bc%89-3\/","title":{"rendered":"Hygromycin B \u6f6e\u9709\u7d20B\uff08\u51bb\u5e72\u7c89\uff09"},"content":{"rendered":"

Hygromycin B \u6f6e\u9709\u7d20B<\/strong><\/span><\/p>\n

\u4ea7\u54c1\u6807\u7b7e<\/strong><\/span><\/p>\n

Hygromycin B\u00a0 \u6f6e\u9709\u7d20B\uff1b50mg\/ml\uff1bHph\u6f6e\u9709\u7d20\u78f7\u9178\u8f6c\u79fb\u9176\uff1b\u9057\u4f20\u9709\u7d20G418\uff1bBlasticidin S \u6740\u7a3b\u761f\u83cc\u7d20\uff08\u706d\u761f\u6563\uff09\uff1bZeocin \u535a\u83b1\u9709\u7d20\uff1bCAS\uff1a31282-04-9<\/span><\/p>\n

\u4ea7\u54c1\u4fe1\u606f<\/strong><\/span><\/p>\n\n\n\n\n\n\n\n
\u4ea7\u54c1\u540d\u79f0\u00a0\u00a0<\/strong><\/span><\/td>\n\u00a0\u4ea7\u54c1\u7f16\u53f7\u00a0<\/strong><\/span><\/td>\n\u00a0\u89c4\u683c\u00a0\u00a0<\/strong><\/span><\/td>\n\u00a0\u4ef7\u683c\uff08\u5143\uff09<\/strong>\u00a0<\/span>\u00a0<\/span><\/td>\n<\/tr>\n
Hygromycin B \u6f6e\u9709\u7d20B\uff08\u51bb\u5e72\u7c89\uff09<\/span><\/td>\n\u00a0MS0003-1G<\/span><\/td>\n\u00a01g<\/span><\/td>\n\u00a0600<\/span><\/td>\n<\/tr>\n
Hygromycin B \u6f6e\u9709\u7d20B\uff08\u51bb\u5e72\u7c89\uff09<\/span><\/td>\n\u00a0MS0003-5G<\/span><\/td>\n\u00a05g<\/span><\/td>\n\u00a02400<\/span><\/td>\n<\/tr>\n
Hygromycin\u00a0 B\u6f6e\u9709\u7d20B\uff08\u51bb\u5e72\u7c89\uff09<\/span><\/td>\n\u00a0MS0003-10G<\/span><\/td>\n\u00a010g<\/span><\/td>\n\u00a03840<\/span><\/td>\n<\/tr>\n
Hygromycin B (50 mg\/ml) \u6f6e\u9709\u7d20B\uff0850 mg\/ml\uff09<\/span><\/td>\n\u00a0MS0003-20ML<\/span><\/td>\n\u00a020ml<\/span><\/td>\n\u00a0720<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u4ea7\u54c1\u63cf\u8ff0<\/strong><\/span><\/p>\n

\u6f6e\u9709\u7d20B\uff08Hygromycin B\uff09\uff0c\u4ece\u5438\u6c34\u94fe\u9709\u83cc\uff08Streptomyces hygroscopicus\uff09\u5206\u79bb\u5230\u7684\u4e00\u79cd\u6c28\u57fa\u7cd6\u82f7\u7c7b\u6297\u751f\u7d20\u3002\u901a\u8fc7\u4e0e30S\u6838\u7cd6\u4f53\u4e9a\u57fa\u7ed3\u5408\uff0c\u8bf1\u5bfc\u5bf9mRNA\u6a21\u677f\u7684\u9519\u8bfb\u4e14\u6291\u5236\u6613\u4f4d\uff0c\u4ece\u800c\u4ee5\u963b\u6b62\u86cb\u767d\u5408\u6210\u3002\u6f6e\u9709\u7d20B\u80fd\u6740\u6b7b\u7ec6\u83cc\u3001\u771f\u83cc\u548c\u9ad8\u7b49\u771f\u6838\u7ec6\u80de\u3002\u4e3b\u8981\u7528\u6765\u7b5b\u9009\u548c\u7ef4\u6301\u57f9\u517b\u7a33\u5b9a\u8f6c\u67d3Hph\u8f7d\u4f53\u7684\u539f\u6838\u7ec6\u80de\u6216\u771f\u6838\u7ec6\u80de\uff08\u690d\u7269\u7ec6\u80de\u548c\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\uff09\u3002<\/span><\/p>\n

\u5bf9\u6f6e\u9709\u7d20B\uff08Hygromycin B\uff09\u7684\u6297\u6027\u6765\u6e90\u4e8e\u5927\u80a0\u6746\u83cc\u6f6e\u9709\u7d20\u6297\u6027\u57fa\u56e0\uff08hph\u6216hyg\uff09\uff0c\u8be5\u57fa\u56e0\u80fd\u7f16\u7801\u6f6e\u9709\u7d20\u78f7\u9178\u8f6c\u79fb\u9176\uff08hygromycin phosphotransferase\uff09\uff0c\u4f7f\u5f97\u6f6e\u9709\u7d20B\u53d1\u751f\u78f7\u9178\u5316\u4ece\u800c\u5931\u53bb\u6d3b\u6027\u3002\u76ee\u524dHph\u6297\u6027\u57fa\u56e0\u4e3b\u8981\u6709\u4e24\u79cd\u6765\u6e90\uff0c\u6700\u521d\u6765\u6e90\u662f\u5927\u80a0\u6746\u83cc\uff08Escherichia coli\uff09\uff0c\u53e6\u4e00\u6765\u6e90\u662f\u6f6e\u9709\u7d20B\u4ea7\u751f\u83cc\u5438\u6c34\u94fe\u9709\u83cc\uff08Streptomyces hygroscopicus\uff09\u3002\u5982\u4eca\uff0c\u643a\u5e26Hph\u6297\u6027\u7684\u8f7d\u4f53\u5927\u591a\u6765\u81ea\u5927\u80a0\u6746\u83cc\u3002<\/span><\/p>\n

\u6211\u53f8\u63d0\u4f9b\u4e24\u79cd\u4ea7\u54c1\u5f62\u5f0f\u7684\u6f6e\u9709\u7d20B\u3002<\/span><\/strong><\/span><\/p>\n

\u4e00\u79cd\u4ee5\u51bb\u5e72\u7c89\u5f62\u5f0f\u63d0\u4f9b\uff0c\u8d27\u53f7\u5206\u522b\u4e3a\uff1aMS0003-1G\uff0cMS0003-5G\uff0cMS0003-10G\u3002\u9700\u8981\u914d\u5236\u6210\u50a8\u5b58\u6db2\u540e\u5907\u7528\u3002<\/span><\/p>\n

\u4e00\u79cd\u4ee5\u6eb6\u4e8ePBS\u7684\u65e0\u83cc\u6db2\u4f53\u5f62\u5f0f\u63d0\u4f9b\uff0c\u8d27\u53f7\u4e3aMS0003-20ML\uff0c\u4f7f\u7528\u66f4\u7b80\u5355\u65b9\u4fbf\u3002<\/span><\/p>\n

\u4ea7\u54c1\u7279\u6027<\/strong><\/span><\/p>\n

1\uff09\u5316\u5b66\u540d\uff1aO-6-Amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene-(1-2-3)-O-\u03b2-D-talopyranosyl(1-5)- 2-deoxy-N3-methyl-D-streptamine<\/span><\/p>\n

2\uff09\u540c\u4e49\u540d\uff1aHygromycin B, from Streptomyces hygroscopicus\u6f6e\u9709\u7d20\u8d28B\uff0c\u6765\u6e90\u4e8e\u5438\u6c34\u94fe\u9709\u83cc<\/span><\/p>\n

3\uff09CAS NO\uff1a31282-04-9<\/span><\/p>\n

4\uff09\u5206\u5b50\u5f0f\uff1aC20H37N3O13<\/span><\/p>\n

5\uff09\u5206\u5b50\u91cf\uff1a527.53g\/mol<\/span><\/p>\n

6\uff09\u00a0\u00a0\u5916\u89c2\uff1a\u7c7b\u767d\u8272\u81f3\u767d\u8272\u7c89\u672b<\/span><\/p>\n

7\uff09\u7eaf\u5ea6\uff1a\u226590%\uff08HPLC\uff09<\/span><\/p>\n

8\uff09\u6548\u4ef7\uff1a\u22651050 U\/mg<\/span><\/p>\n

9\uff09\u5316\u5b66\u7ed3\u6784\uff1a\"\"<\/span><\/p>\n

\u4fdd\u5b58\u4e0e\u8fd0\u8f93\u65b9\u6cd5<\/strong><\/span><\/p>\n

\u4fdd\u5b58\uff1a\u7c89\u672b-20\u2103\u5e72\u71e5\u4fdd\u5b58\uff0c2\u5e74\u6709\u6548\u3002\u6eb6\u6db22-8\u00b0C\u907f\u5149\u4fdd\u5b58\uff0c1\u5e74\u6709\u6548\u3002<\/span><\/p>\n

\u8fd0\u8f93\uff1a\u51b0\u888b\u8fd0\u8f93\u3002<\/span><\/p>\n

\u4f7f\u7528\u65b9\u6cd5<\/strong><\/span><\/p>\n

1. \u50a8\u5b58\u6db2\u5236\u5907<\/strong><\/span><\/p>\n

1\uff09\u6f6e\u9709\u7d20B\uff08\u7c89\u672b\u5f62\u5f0f\uff09\uff1a\u79f0\u53d6\u9002\u91cf\u7c89\u672b\u6eb6\u4e8e1\u00d7PBS\u914d\u5236\u621050mg\/ml\u6eb6\u6db2\uff0c\u75280.22\u03bcm\u6ee4\u819c\u8fc7\u6ee4\u9664\u83cc\uff0c\u7f6e\u4e8e2-8\u00b0C\u907f\u5149\u4fdd\u5b58\u3002<\/span><\/p>\n

2\uff09\u6f6e\u9709\u7d20B\uff08\u6eb6\u6db2\u5f62\u5f0f\uff09\uff1a\u5df2\u662f\u65e0\u83cc\u50a8\u5b58\u6db2\uff0c\u76f4\u63a5\u7528\u7ec6\u80de\u57f9\u517b\u6db2\u7a00\u91ca\u5230\u5de5\u4f5c\u6d53\u5ea6\u5373\u53ef\u4f7f\u7528\u3002<\/span><\/p>\n

2. \u5efa\u8bae\u5de5\u4f5c\u6d53\u5ea6<\/strong><\/span><\/p>\n

\u6f6e\u9709\u7d20B\u7528\u6765\u7b5b\u9009\u7a33\u5b9a\u8f6c\u67d3\u7ec6\u80de\u682a\u7684\u5de5\u4f5c\u6d53\u5ea6\u9700\u8981\u6839\u636e\u7ec6\u80de\u7c7b\u578b\uff0c\u57f9\u517b\u57fa\uff0c\u751f\u957f\u6761\u4ef6\u548c\u7ec6\u80de\u4ee3\u8c22\u7387\u800c\u53d8\u5316\u3002<\/span><\/p>\n

1\uff09\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\u4f7f\u7528\u6d53\u5ea6\u4e3a50-1000 \u03bcg\/ml\uff0c\u5e38\u7528\u7b5b\u9009\u6d53\u5ea6\u4e3a200 \u03bcg\/ml\uff0c\u6700\u4f73\u6d53\u5ea6\u9700\u8981\u6740\u706d\u66f2\u7ebf\u6765\u786e\u5b9a\u3002<\/span><\/p>\n

2\uff09\u690d\u7269\u7ec6\u80de\u5e38\u7528\u6d53\u5ea6\uff1a20-200 \u03bcg\/ml\uff1b<\/span><\/p>\n

3\uff09\u7ec6\u83cc\u5e38\u7528\u6d53\u5ea6\uff1a20-200 \u03bcg\/ml\uff1b<\/span><\/p>\n

4\uff09\u771f\u83cc\u5e38\u7528\u6d53\u5ea6\uff1a200-1000 \u03bcg\/ml\uff1b<\/span><\/p>\n

3. \u6740\u706d\u66f2\u7ebf\u7684\u5efa\u7acb<\/strong><\/span><\/p>\n

\u5efa\u8bae\u521d\u6b21\u5b9e\u9a8c\u4e00\u5b9a\u8981\u5efa\u7acb\u6740\u706d\u66f2\u7ebf\uff08kill curve\uff09\uff0c\u81f3\u5c11\u8bbe\u7acb5\u4e2a\u6d53\u5ea6\uff0c\u7b5b\u9009\u5230\u9002\u5408\u81ea\u8eab\u5b9e\u9a8c\u4f53\u7cfb\u7684\u6700\u4f73\u6d53\u5ea6\u3002<\/span><\/p>\n

1\uff09\u5c06\u672a\u8f6c\u5316\u7ec6\u80de\u6309\u716720-25%\u7684\u7ec6\u80de\u5bc6\u5ea6\u94fa\u5728\u5408\u9002\u7684\u57f9\u517b\u677f\u4e0a\uff0c\u94fa\u8db3\u591f\u7684\u5b54\u4ee5\u4fdd\u8bc1\u540e\u7eed\u7684\u68af\u5ea6\u5b9e\u9a8c\u300237\u2103\u57f9\u517b\u8fc7\u591c\uff1b\u3010\u6ce8\u610f\u3011\uff1a<\/strong>\u5bf9\u4e8e\u9700\u8981\u66f4\u9ad8\u5bc6\u5ea6\u4ee5\u4fdd\u8bc1\u6d3b\u529b\u7684\u7ec6\u80de\uff0c\u589e\u52a0\u63a5\u79cd\u91cf\u3002<\/span><\/p>\n

2\uff09\u7b2c\u4e8c\u65e5\uff0c\u7528\u542b\u68af\u5ea6\u6d53\u5ea6\u6f6e\u9709\u7d20B\u7684\u57f9\u517b\u57fa\u66ff\u6362\u65e7\u57f9\u517b\u57fa\u3002\u6bd4\u5982\uff0c\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\u5e38\u7528\u6d53\u5ea6\u4e3a50-1000\u03bcg\/ml\uff0c\u53ef\u8bbe50, 100, 250, 500, 750, 1000\u03bcg\/m\u8fd96\u4e2a\u6d53\u5ea6\uff0c\u54043\u4e2a\u5e73\u884c\u3002\u540c\u65f6\u8bbe\u7f6e\u4e00\u9634\u6027\u5bf9\u7167\uff0c3\u4e2a\u5e73\u884c\u3002<\/span><\/p>\n

3\uff09\u63a5\u4e0b\u6765\u6bcf3-4\u65e5\u66f4\u6362\u65b0\u9c9c\u7684\u542b\u6297\u751f\u7d20\u57f9\u517b\u57fa\u3002<\/span><\/p>\n

4\uff09\u6309\u7167\u56fa\u5b9a\u5468\u671f\uff08\u5982\u6bcf2\u5929\uff09\u8fdb\u884c\u6d3b\u7ec6\u80de\u8ba1\u6570\u6765\u786e\u5b9a\u963b\u6b62\u672a\u8f6c\u5316\u7ec6\u80de\u751f\u957f\u7684\u6070\u5f53\u6d53\u5ea6\u3002\u9009\u62e9\u5728\u7406\u60f3\u5929\u6570\uff08\u901a\u5e38\u662f7-10\u5929\uff09\u5185\u80fd\u591f\u6740\u6b7b\u7edd\u5927\u591a\u6570\u7ec6\u80de\u7684\u6700\u4f4e\u6d53\u5ea6\u4e3a\u7a33\u5b9a\u8f6c\u67d3\u7ec6\u80de\u7b5b\u9009\u6240\u9700\u7684\u5de5\u4f5c\u6d53\u5ea6\u3002<\/span><\/p>\n

4\u3001\u7a33\u8f6c\u7ec6\u80de\u7684\u7b5b\u9009<\/strong><\/span><\/p>\n

1\uff09\u8f6c\u67d348h\u540e\uff0c\u7528\u542b\u6700\u4f73\u7b5b\u9009\u6d53\u5ea6\u6f6e\u9709\u7d20B\u7684\u57f9\u517b\u57fa\u8fdb\u884c\u7ec6\u80de\u4f20\u4ee3\u3002\u3010\u6ce8\u610f\u3011\uff1a<\/strong>\u7ec6\u80de\u5904\u4e8e\u6d3b\u8dc3\u5206\u88c2\u72b6\u6001\u65f6\u6297\u751f\u7d20\u7684\u6740\u4f24\u6548\u679c\u6700\u597d\u3002\u5f53\u7ec6\u80de\u5bc6\u5ea6\u8fc7\u9ad8\uff0c\u7b5b\u9009\u6548\u7387\u4f1a\u964d\u4f4e\u3002\u4e3a\u4e86\u5f97\u5230\u8f83\u597d\u7684\u7b5b\u9009\u6548\u679c\uff0c\u5efa\u8bae\u5c06\u7ec6\u80de\u7a00\u91ca\u81f3\u4e30\u5ea6\u4e0d\u8d85\u8fc725%\u3002<\/span><\/p>\n

2\uff09\u6bcf3-4\u65e5\u66f4\u6362\u65b0\u9c9c\u7684\u542b\u6297\u751f\u7d20\u57f9\u517b\u57fa\u3002<\/span><\/p>\n

3\uff09\u7b5b\u90097\u5929\u540e\u89c2\u5bdf\u5e76\u8bc4\u4f30\u7ec6\u80de\u96c6\u843d\u7684\u5f62\u6210\u60c5\u51b5\u3002\u96c6\u843d\u7684\u5f62\u6210\u53ef\u80fd\u8fd8\u9700\u8981\u4e00\u5468\u6216\u8005\u66f4\u591a\u7684\u65f6\u95f4\uff0c\u8fd9\u4e9b\u53d6\u51b3\u4e8e\u5bbf\u4e3b\u7ec6\u80de\u7c7b\u578b\uff0c\u8f6c\u67d3\uff0c\u4ee5\u53ca\u7b5b\u9009\u6548\u679c\u3002<\/span><\/p>\n

4\uff09\u6311\u53d65-10\u4e2a\u6297\u6027\u514b\u9686\u523035mm\u7ec6\u80de\u57f9\u517b\u677f\uff0c\u7ee7\u7eed\u7528\u542b\u836f\u7269\u7684\u7b5b\u9009\u57f9\u517b\u6db2\u7ef4\u6301\u57f9\u517b7\u5929\u3002<\/span><\/p>\n

5\uff09\u4e4b\u540e\u6362\u7528\u6b63\u5e38\u57f9\u517b\u57fa\u57f9\u517b\u5373\u53ef\u3002<\/span><\/p>\n

\u6ce8\u610f\u4e8b\u9879<\/strong><\/span><\/p>\n

\u4e3a\u4e86\u60a8\u7684\u5b89\u5168\u548c\u5065\u5eb7\uff0c\u8bf7\u7a7f\u5b9e\u9a8c\u670d\u5e76\u6234\u4e00\u6b21\u6027\u624b\u5957\u64cd\u4f5c\u3002<\/span><\/p>\n

\u00a0\u2014 \u2014 Written\/Edited by V. Shallan\u3010\u7248\u6743\u5f52\u96c6\u5947\u751f\u7269\/MKBio\u61cb\u5eb7\u6240\u6709\u3011<\/span><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u66f4\u591a\u4ea7\u54c1\u4fe1\u606f\u8bf7\u54a8\u8be2\u5ba2\u670d\uff01<\/p>\n","protected":false},"featured_media":26239,"template":"","meta":[],"product_cat":[99],"product_tag":[102],"_links":{"self":[{"href":"\/\/www.5080666.com\/wp-json\/wp\/v2\/product\/299"}],"collection":[{"href":"\/\/www.5080666.com\/wp-json\/wp\/v2\/product"}],"about":[{"href":"\/\/www.5080666.com\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"\/\/www.5080666.com\/wp-json\/wp\/v2\/media\/26239"}],"wp:attachment":[{"href":"\/\/www.5080666.com\/wp-json\/wp\/v2\/media?parent=299"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"\/\/www.5080666.com\/wp-json\/wp\/v2\/product_cat?post=299"},{"taxonomy":"product_tag","embeddable":true,"href":"\/\/www.5080666.com\/wp-json\/wp\/v2\/product_tag?post=299"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}