{"id":17646,"date":"2019-09-03T16:53:04","date_gmt":"2019-09-03T08:53:04","guid":{"rendered":"\/\/www.5080666.com\/?post_type=product&p=17646"},"modified":"2022-02-09T19:39:54","modified_gmt":"2022-02-09T11:39:54","slug":"hypoxyprobe-hypoxyprobetm-1-plus-kit-%e7%bc%ba%e6%b0%a7%e6%8e%a2%e9%92%88%e6%a3%80%e6%b5%8b%e8%af%95%e5%89%82%e7%9b%92","status":"publish","type":"product","link":"\/\/www.5080666.com\/product\/hypoxyprobe-hypoxyprobetm-1-plus-kit-%e7%bc%ba%e6%b0%a7%e6%8e%a2%e9%92%88%e6%a3%80%e6%b5%8b%e8%af%95%e5%89%82%e7%9b%92\/","title":{"rendered":"Hypoxyprobe HypoxyprobeTM-1 Plus Kit \u7f3a\u6c27\u63a2\u9488\u68c0\u6d4b\u8bd5\u5242\u76d2"},"content":{"rendered":"

HypoxyprobeTM<\/sup>-1 Plus Kit \u7f3a\u6c27\u63a2\u9488\u68c0\u6d4b\u8bd5\u5242\u76d2<\/strong><\/span><\/p>\n

 <\/p>\n

\u641c\u7d22\u5173\u952e\u8bcd\uff1a<\/strong><\/span><\/p>\n

Hypoxyprobe, Inc.\uff08\u7f3a\u6c27\u63a2\u9488\u516c\u53f8\uff09\uff1bPimonidazole HCl\uff1b\u7f3a\u6c27\u6807\u5fd7\u7269\uff08Hypoxia maker\uff09\uff1bChemicon Inc.\uff1b<\/span><\/p>\n

\u4ea7\u54c1\u5e94\u7528\uff1a<\/strong><\/span><\/p>\n

\u514d\u75ab\u5316\u5b66\u6cd5\u68c0\u6d4b\u7ec6\u80de\u548c\u7ec4\u7ec7\u7f3a\u6c27\u60c5\u51b5\uff0c\u5305\u62ec\u514d\u75ab\u8367\u5149\uff08IF\uff09\uff0c\u514d\u75ab\u8fc7\u6c27\u5316\u7269\u9176\uff0cWB\u6216\u6d41\u5f0f\u7ec6\u80de\u5206\u6790\uff08FC\uff09\uff1b\u5f53HypoxyprobeTM-1 Plus Kit\u7ed3\u5408anti-FITC\u6297\u4f53\u68c0\u6d4b\u5c0f\u9f20\u7ec4\u7ec7\uff0c\u67d3\u8272\u80cc\u666f\u6781\u5176\u4f4e\u3002<\/span><\/p>\n

\u8bd5\u5242\u76d2\u7ec4\u5206\uff1a<\/strong><\/span><\/p>\n\n\n\n\n\n\n\n
\u7f16\u53f7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/strong><\/span><\/td>\n\u7ec4\u5206\u540d\u79f0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/strong><\/span><\/td>\n\n

\u8d27\u53f7\uff08\u89c4\u683c\uff09<\/strong><\/span><\/p>\n<\/td>\n

\u4fdd\u5b58\u65b9\u6cd5<\/strong>\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/td>\n<\/tr>\n
HP2-100Kit\u00a0\u00a0\u00a0 <\/strong><\/span><\/td>\nHP2-200Kit\u00a0\u00a0\u00a0 <\/strong><\/span><\/td>\nHP2-1000Ki<\/strong>t<\/strong>\u00a0\u00a0<\/span><\/td>\n<\/tr>\n
1<\/span><\/td>\nPimonidazole HCl<\/span><\/td>\n100mg<\/span><\/td>\n200mg<\/span><\/td>\n1000mg<\/span><\/td>\n\u5ba4\u6e29\u62162-8\u2103\u907f\u5149<\/span><\/td>\n<\/tr>\n
2<\/span><\/td>\n4.3.11.3 mouse FITC-MAb<\/span><\/td>\n200\u03bcl<\/span><\/td>\n200\u03bcl<\/span><\/td>\n2\u00d7200\u03bcl<\/span><\/td>\n2-8\u2103\u907f\u5149\uff0c\u907f\u514d\u53cd\u590d\u51bb\u878d<\/span><\/td>\n<\/tr>\n
3<\/span><\/td>\nRabbit anti-FITC HRP<\/span><\/td>\n200\u03bcl<\/span><\/td>\n200\u03bcl<\/span><\/td>\n2\u00d7200\u03bcl<\/span><\/td>\n2-8\u2103\u907f\u5149\uff0c\u907f\u514d\u53cd\u590d\u51bb\u878d<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\u8bd5\u5242\u76d2\u7ec4\u5206\u8be6\u7ec6\u63cf\u8ff0\uff1a<\/strong><\/span><\/p>\n

1\u3001<\/strong>Hypoxyprobe-1<\/strong><\/span><\/p>\n

Hypoxyprobe-1\u662f2-\u785d\u57fa\u54aa\u5511\u7684\u66ff\u4ee3\u7269\uff0c\u5176\u5316\u5b66\u540d\u548c\u552f\u4e00\u6210\u5206\u662fPimonidazole hydrochloride\uff08Pimonidazole HCl\uff09\uff0c\u5206\u5b50\u91cf\u4e3a290.8\uff0c\u6c34\u6eb6\u6027\u4e3a400mM\uff08=116 mg\/ml\uff09\uff0c\u7d2b\u5916\u5438\u6536\uff08324nm\uff09\u7684\u5438\u5149\u7cfb\u6570\u4e3a7020\uff080.9%\u751f\u7406\u76d0\u6c34\uff09\u3002<\/span><\/p>\n

Hypoxyprobe-1\u7684\u7c89\u672b\u548c\u6eb6\u6db2\u5f62\u5f0f\u7686\u5177\u5316\u5b66\u7a33\u5b9a\u6027\u3002\u6bd4\u5982\uff0c\u56fa\u4f53\u5f62\u5f0f\u7684Hypoxyprobe-1\u4e8e\u67d4\u5f31\u5149\u7ebf\u4e0b\u5ba4\u6e29\u4fdd\u5b582\u5e74\uff0cUV\u548cHPLC\u5206\u6790\u6cd5\u8bc4\u4f30\u65e0\u4efb\u4f55\u964d\u89e3\u53d1\u751f\u3002\u6eb6\u4e8e0.9%\u751f\u7406\u76d0\u6c34\u7684Hypoxyprobe-1\uff0c\u6d53\u5ea6100g\/L\u7f6e\u4e8e2-8\u2103\u4fdd\u5b584.5\u5e74\uff0cUV\u548cHPLC\u5206\u6790\u6cd5\u8bc4\u4f30\u65e0\u4efb\u4f55\u964d\u89e3\u53d1\u751f\u3002<\/span><\/p>\n

\u4f5c\u7528\u673a\u7406\uff1a\u7f3a\u6c27\u7ec6\u80de\u4e2dPimonidazole\u88ab\u8fd8\u539f\u540e\u5f97\u4ee5\u6d3b\u5316\u3002\u8fd9\u4e00\u6d3b\u5316\u7684\u4e2d\u95f4\u7269\u4e0e\u86cb\u767d\u8d28\u3001\u591a\u80bd\u548c\u6c28\u57fa\u9178\u4e2d\u7684\u5def\u57fa\u57fa\u56e2\uff08thiol groups\uff09\u5f62\u6210\u7a33\u5b9a\u7684\u52a0\u5408\u7269\u3002Mab1\u6297\u4f53\u7ed3\u5408\u8fd9\u4e9b\u52a0\u5408\u7269\u4ece\u800c\u80fd\u501f\u7531\u514d\u75ab\u5316\u5b66\u6cd5\u6765\u68c0\u6d4b\u3002<\/span><\/p>\n

 <\/p>\n

2\u3001FITC-MAb1\u6297\u4f53<\/strong><\/span><\/p>\n

FITC-MAb1\u662f\u8367\u5149\u7d20\u5076\u8054\u7684\u5c0f\u9f20IgG1\u5355\u514b\u9686\u6297\u4f53\uff08\u514b\u96864.3.11.3\uff09\uff0c\u6d53\u5ea6\u4e3a0.5mg\/ml\uff08in PBS\uff09\u3002<\/span><\/p>\n

\u611f\u5174\u8da3\u7ec4\u7ec7\u53ef\u7528\u514d\u75ab\u7ec4\u5316\u68c0\u6d4b\uff08IHC\uff09\uff0c\u51b0\u51bb\u5207\u7247\u6216\u8005\u798f\u5c14\u9a6c\u6797\u56fa\u5b9a\u7684\u77f3\u8721\u5305\u57cb\u5207\u7247\uff1b\u4e5f\u53ef\u7ec4\u7ec7\u964d\u89e3\u540e\u7528\u6d41\u5f0f\u5206\u6790\u6cd5\u68c0\u6d4b\uff1b<\/span><\/p>\n

3\u3001anti-FITC HRP\u6297\u4f53<\/strong><\/span><\/p>\n

\u672c\u6297\u4f53\u4e3a\u5076\u8054\u5230HRP\u7684\u5154IgG\u591a\u514b\u9686\u6297\u4f53\u3002\u7528\u4e8e\u68c0\u6d4b\u798f\u5c14\u9a6c\u6797\u77f3\u8721\u5305\u57cb\u7684\u5c0f\u9f20\u80bf\u7624\u7ec4\u7ec7\uff0c\u4e0d\u4ec5\u6709\u5f3a\u8367\u5149\u4fe1\u53f7\uff0c\u4e14\u80cc\u666f\u6781\u5176\u4f4e\u3002<\/span><\/p>\n

 <\/p>\n

\u4f7f\u7528\u6307\u5357\uff1a<\/strong><\/span><\/p>\n

1\u3001Animal Studies \u52a8\u7269\u7814\u7a76<\/strong><\/span><\/p>\n

\u7814\u7a76\u6b63\u5e38\u6216\u80bf\u7624\u7ec4\u7ec7\u7f3a\u6c27\u9996\u5148\u901a\u8fc7\u9759\u8109\u6ce8\u5c04\uff08intravenous\u00a0infusion\uff09\u3001\u8179\u8154\u6ce8\u5c04\uff08intraperitoneal injection\uff09\u6216\u53e3\u670d\uff08oral ingestion\uff09\u6cd5\u7ed9Hypoxyprobe-1\u6eb6\u6db2\uff0c\u5242\u91cf\u4e3a60mg\/kg\u4f53\u91cd\u3002\u6bd4\u5982\uff0c25g\u5c0f\u9f20\u7684\u63a2\u9488\u7528\u91cf\u4e3a1.5mg\/\u53ea\u5c0f\u9f20\u3002\uff08\u9ad8\u8fbe400mg\/kg\u7684\u5242\u91cf\u7528\u4e8e\u5c0f\u9f20\u65e0\u53ef\u89c1\u6bd2\u6027\u6216\u5f15\u8d77\u7ec4\u7ec7\u7f3a\u6c27\u53d8\u5316\uff0c\u4f4660mg\/kg\u4ee5\u6700\u4f4e\u6210\u672c\u7ed9\u4e88\u826f\u597d\u7684\u67d3\u8272\u7ed3\u679c\uff09\u3002<\/span><\/p>\n

\u7ed9\u836f\u540e\uff0cHypoxyprobe-1\u5206\u5e03\u5230\u5305\u62ec\u5927\u8111\u7684\u6240\u6709\u7ec4\u7ec7\uff0c\u4e14\u4ec5\u4ec5\u5728\u6c27\u6d53\u5ea6\u226414\u03bcM\uff08\u76f8\u5f53\u4e8epO2=10mm Hg\uff0c37\u2103\uff09\u7684\u7ec6\u80de\u5185\u4e0e\u86cb\u767d\u8d28\u5def\u57fa\u5f62\u6210\u52a0\u5408\u7269\u3002\u9664\u4e86\u80bf\u7624\u4e4b\u5916\uff0c\u6b63\u5e38\u7ec4\u7ec7\u5982\u809d\u810f\u3001\u80be\u810f\u548c\u76ae\u80a4\u542bpO2\u7b49\u4e8e\u6216\u4f4e\u4e8e10mm Hg\u7684\u7ec6\u80de\uff0c\u4e5f\u4f1a\u7ed3\u5408Hypoxyprobe-1\u3002<\/span><\/p>\n

 <\/p>\n

2\u3001 Tissue cultures \u7ec4\u7ec7\u57f9\u517b<\/strong><\/span><\/p>\n

\u901a\u5e38\u60c5\u51b5\u4e0b\uff0c\u7ec6\u80de\u60ac\u6db2\u7f6e\u4e8e\u542b100-200\u03bcM Hypoxyprobe-1\u7684\u7f3a\u6c27\u73af\u5883\u4e2d\u5b75\u80b21-2h\u3002\u4e4b\u540e\u7ec6\u80de\u79bb\u5fc3\u6d82\u7247\u3001\u56fa\u5b9a\u3001FTIC-MAb1\u548canti-FITC chromogenic secondary\u67d3\u8272\u3002<\/span><\/p>\n

 <\/p>\n

\u8ba2\u8d2d\u4fe1\u606f\uff1a<\/strong>\u539f\u88c5\u6b63\u54c1\uff0c\u73b0\u8d27\u4f9b\u5e94\u3002<\/strong>\u6b22\u8fce\u6765\u7535\u54a8\u8be2\uff0c\u7535\u8bdd\uff1a021-54736159\uff0c18121428569\uff1bQQ\uff1a3308240863\uff0c2971634497\u3002<\/strong><\/span><\/p>\n\n\n\n\n\n\n
\u8d27\u53f7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/strong><\/span><\/td>\n\u540d\u79f0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/strong><\/span><\/td>\n\u76ee\u5f55\u4ef7\uff08\u5143\uff09\u00a0 <\/strong><\/span><\/td>\n\u8d27\u671f\u00a0\u00a0\u00a0\u00a0\u00a0<\/strong>\u00a0\u00a0\u00a0\u00a0<\/span><\/td>\n<\/tr>\n
HP2-100Kit<\/span><\/td>\nHypoxyprobe Plus Kit (100 mg pimonidazole HCl plus 1 unit of 4.3.11.3 mouse<\/span><\/p>\n

FITC-MAb and 1 unit of anti-FITC HRP)<\/span><\/td>\n

5280<\/span><\/td>\n\u73b0\u8d27<\/span><\/td>\n<\/tr>\n
HP2-200Kit<\/span><\/td>\nHypoxyprobe Plus Kit (100 mg pimonidazole HCl plus 1 unit of 4.3.11.3 mouse<\/span><\/p>\n

FITC-MAb and 1 unit of anti-FITC HRP)<\/span><\/td>\n

9360<\/span><\/td>\n\u54a8\u8be2<\/span><\/td>\n<\/tr>\n
HP2-1000Kit<\/span><\/td>\nHypoxyprobe Plus Kit (1000 mg pimonidazole HCl plus 2 units of 4.3.11.3 mouse<\/span><\/p>\n

FITC-MAb and 2 units of anti-FITC HRP)<\/span><\/td>\n

16560<\/span><\/td>\n\u54a8\u8be2<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\u00a0\u2014 \u2014Written\/Edited by V. Shallan\u3010\u7248\u6743\u5f52\u96c6\u5947\u751f\u7269\/MKBio\u61cb\u5eb7\u6240\u6709\u3011<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

\u66f4\u591a\u4ea7\u54c1\u4fe1\u606f\u8bf7\u54a8\u8be2\u5ba2\u670d\uff01<\/p>\n","protected":false},"featured_media":27793,"template":"","meta":[],"product_cat":[220],"product_tag":[228,182],"_links":{"self":[{"href":"\/\/www.5080666.com\/wp-json\/wp\/v2\/product\/17646"}],"collection":[{"href":"\/\/www.5080666.com\/wp-json\/wp\/v2\/product"}],"about":[{"href":"\/\/www.5080666.com\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"\/\/www.5080666.com\/wp-json\/wp\/v2\/media\/27793"}],"wp:attachment":[{"href":"\/\/www.5080666.com\/wp-json\/wp\/v2\/media?parent=17646"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"\/\/www.5080666.com\/wp-json\/wp\/v2\/product_cat?post=17646"},{"taxonomy":"product_tag","embeddable":true,"href":"\/\/www.5080666.com\/wp-json\/wp\/v2\/product_tag?post=17646"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}