{"id":29328,"date":"2021-09-13T15:07:39","date_gmt":"2021-09-13T07:07:39","guid":{"rendered":"\/\/www.5080666.com\/?p=29328"},"modified":"2022-02-21T15:22:56","modified_gmt":"2022-02-21T07:22:56","slug":"cell-tracker%e7%bb%86%e8%83%9e%e7%a4%ba%e8%b8%aa-%e7%bb%86%e8%83%9e%e8%bf%bd%e8%b8%aa%e8%8d%a7%e5%85%89%e6%8e%a2%e9%92%88%e4%b8%93%e9%a2%98","status":"publish","type":"post","link":"\/\/www.5080666.com\/tech-features\/29328\/","title":{"rendered":"Cell-Tracker\u7ec6\u80de\u793a\u8e2a\/\u7ec6\u80de\u8ffd\u8e2a\u8367\u5149\u63a2\u9488\u4e13\u9898"},"content":{"rendered":"

\u5173\u952e\u4fe1\u606f\uff1a<\/b><\/strong><\/span><\/p>\n

Cell-Tracker\u7cfb\u5217\u793a\u8e2a\u63a2\u9488\uff08\u7eff\u8272\u3001\u6a59\u8272\u3001\u7ea2\u8272\u3001\u6df1\u7ea2\u8272\uff0c\u591a\u91cd\u6807\u8bb0\uff1b\u77ed\u4e2d\u671f\u793a\u8e2a\uff1b\uff09<\/span><\/p>\n

\u4e13\u9898\u6807\u7b7e\uff1a<\/b><\/strong><\/span><\/p>\n

Cell Tracking \u7ec6\u80de\u793a\u8e2a\uff1bCell movement \u7ec6\u80de\u8fd0\u52a8\uff1bCell-Tracker Green CMFDA\uff1bCell-Tracker Orange CMTMR\uff1bCell-Tracker Red CMTPX\uff1bCell-Tracker Deep Red\uff1b<\/span><\/p>\n

\u80cc\u666f\u9700\u6c42\uff1a<\/b><\/strong><\/span><\/p>\n

\u7ec6\u80de\u8fd0\u52a8\u548c\u5b9a\u4f4d\u7814\u7a76\u9700\u8981\u4e13\u95e8\u7684\u63a2\u9488\u67d3\u6599\uff0c\u9996\u5148\u5fc5\u987b\u8981\u5bf9\u6d3b\u7ec6\u80de\u65e0\u6bd2\u6027\uff0c\u8fd8\u8981\u540c\u65f6\u5177\u6709\u591a\u79cd\u8367\u5149\u989c\u8272\u53ef\u4f9b\u9009\u62e9\uff0c\u4ee5\u4fbf\u4e0e\u4e0d\u540c\u7684\u4eea\u5668\u6fc0\u5149\u6ce2\u957f\u548c\u6ee4\u5149\u7247\u5339\u914d\u5e76\u80fd\u4e0e\u6297\u4f53\u6807\u8bb0\u6216\u5176\u4ed6\u7ec6\u80de\u5206\u6790\u6807\u8bb0\u63a2\u9488\u5171\u67d3\u8272\u3002<\/span><\/p>\n

\u68c0\u6d4b\u7ec6\u80de\u8fd0\u52a8\u548c\u5b9a\u4f4d\u7684\u6700\u7b80\u5355\u65b9\u6cd5\u5c31\u662f\u4f7f\u7528\u5177\u6709\u819c\u901a\u900f\u6027\u7684\u63a2\u9488\u67d3\u6599\u8fdb\u5165\u7ec6\u80de\u5e76\u5728\u52a0\u8f7d\u540e\u8f6c\u53d8\u4e3a\u819c\u975e\u901a\u900f\u6027\u7684\u4ea7\u7269\u3002\u7406\u60f3\u7684\u7ec6\u80de\u793a\u8e2a\u67d3\u6599\u9700\u8981\u53ef\u5b58\u7559\u4e8e\u5206\u88c2\u591a\u4ee3\u540e\u7684\u5b50\u7ec6\u80de\u4e2d\uff0c\u4f46\u4e0d\u4f1a\u901a\u8fc7\u63a5\u89e6\u8f6c\u79fb\u5230\u76f8\u90bb\u7ec6\u80de\u4e2d\u3002Cell-Tracker\u7cfb\u5217\u793a\u8e2a\u67d3\u6599\u5c31\u662f\u5728\u8fd9\u6837\u7684\u80cc\u666f\u4e0b\u5f00\u53d1\u800c\u6765\uff1a\u5728\u5de5\u4f5c\u6d53\u5ea6\u4e0b\u4fdd\u6301\u7a33\u5b9a\u4e14\u65e0\u7ec6\u80de\u6bd2\u6027\uff0c\u53ef\u7a33\u5b9a\u5b58\u7559\u5728\u7ec6\u80de\u4e2d\uff0c\u4e14\u5728\u751f\u7406 pH \u6761\u4ef6\u4e0b\u53ef\u53d1\u51fa\u660e\u4eae\u7684\u8367\u5149\u3002Cell-Tracker\u793a\u8e2a\u8367\u5149\u67d3\u6599\u8fd8\u5177\u6709\u591a\u79cd\u53d1\u5c04\u5149\u8c31\uff0c\u53ef\u8f7b\u677e\u4e0e\u6297\u4f53\u6807\u8bb0\u7ed3\u5408\u4f7f\u7528\u3002<\/span><\/span><\/p>\n

 <\/p>\n

\u4ea7\u54c1\u63cf\u8ff0\uff1a<\/b><\/strong><\/span><\/p>\n

Cell-Tracker\u8367\u5149\u63a2\u9488\u662f\u7528\u6765\u76d1\u6d4b\u7ec6\u80de\u8fd0\u52a8\u3001\u5b9a\u4f4d\u3001\u589e\u6b96\u3001\u8fc1\u79fb\u3001\u8d8b\u5316\u548c\u4fb5\u88ad\u7684\u4f18\u79c0\u5de5\u5177\u3002<\/b><\/strong><\/span><\/p>\n

Cell-Tracker\u8367\u5149\u63a2\u9488<\/b><\/strong>\u80fd\u81ea\u7531\u7a7f\u900f\u7ec6\u80de\u819c\u8fdb\u5165\u7ec6\u80de\uff0c\u5728\u80de\u5185\u8f6c\u5316\u751f\u6210\u4e0d\u5177\u7ec6\u80de\u819c\u6e17\u900f\u6027\u7684\u53cd\u5e94\u4ea7\u7269\u3002Cell-Tracker\u8367\u5149\u63a2\u9488\uff08\u9664\u4e86Cell-Tracker Deep Red\uff09\u542b\u6709\u4e00\u6c2f\u7532\u57fa\u6216\u6eb4\u7532\u57fa\u57fa\u56e2\uff0c\u4e0e\u5def\u57fa\u53cd\u5e94\uff0c\u5229\u7528\u8c37\u80f1\u7518\u80bdS\u8f6c\u79fb\u9176\u4ecb\u5bfc\u7684\u50ac\u5316\u4f5c\u7528\u3002\u5927\u591a\u6570\u7ec6\u80de\u4e2d\u8c37\u80f1\u7518\u80bd\u542b\u91cf\u5f88\u9ad8\uff08\u9ad8\u8fbe10mM\uff09\uff0c\u8c37\u80f1\u7518\u80bd\u8f6c\u79fb\u9176\u666e\u904d\u5b58\u5728\u3002Cell-Tracker Deep Red\u542b\u4e00\u7425\u73c0\u9170\u4e9a\u80fa\u916f\u53cd\u5e94\u57fa\u56e2\uff0c\u4e0e\u86cb \u767d\u4e0a\u7684\u80fa\u57fa\u53cd\u5e94\u3002\u4e00\u65e6\u8f6c\u5316\u4e3a\u4e0d\u53ef\u9006\u4ea7\u7269\uff0c\u8fd9\u4e9b\u4ea7\u7269\u51e0\u6b21\u4f20\u4ee3\u90fd\u80fd\u826f\u597d\u7684\u4fdd\u7559\u5728\u6d3b\u7ec6\u80de\u4e2d\u3002\u5728\u7ec6\u80de\u7fa4\u5185\uff0c\u67d3\u6599\u53ea\u4f1a\u8f6c\u79fb\u5230\u5b50\u4ee3\u7ec6\u80de\uff0c\u4e0d\u4f1a\u8f6c\u79fb\u5230\u90bb\u8fd1\u7ec6\u80de\u3002<\/span><\/p>\n

Cell-Tracker\u63a2\u9488<\/b><\/strong>\u7279\u5730\u8bbe\u8ba1\u4f7f\u5176\u81f3\u5c1172h\uff08\u5178\u578b\u67093~6\u4ee3\uff09\u80fd\u5c55\u793a\u8367\u5149\uff0c\u6b64\u67d3\u6599\u8868\u73b0\u51fa\u7406\u60f3\u7684\u793a\u8e2a\u7279\u5f81\uff1a\u7a33\u5b9a\u3001\u5de5\u4f5c\u6d53\u5ea6\u4e0b\u65e0\u6bd2\u6027\u3001\u826f\u597d\u4fdd\u7559\u5728\u7ec6\u80de\uff0c\u4e14\u5728\u751f\u7406pH\u4e0b\u5448\u660e\u4eae\u8367\u5149\u3002<\/span><\/p>\n

 <\/p>\n

\u91cd\u8981\u7279\u5f81\uff1a<\/b><\/strong><\/span><\/p>\n

\u2460\u00a0\u53ef\u5feb\u901f\u52a0\u8f7d\u6807\u8bb0\uff08\u901a\u5e38 15 \u5206\u949f\u81f3 1 \u5c0f\u65f6\uff09<\/span><\/p>\n

\u2461\u00a0\u77ed\u671f\u8ffd\u8e2a\uff08\u4e00\u822c 3-6\u4ee3\u621672h\uff09<\/span><\/p>\n

\u2462\u00a0\u5177\u6709\u8f83\u5f3a\u7684\u8010\u5149\u6f02\u767d\/\u6297\u5149\u6dec\u706d\u7279\u6027<\/span><\/p>\n

\u2463\u00a0\u8367\u5149\u4fe1\u53f7\u4eae\u5ea6\u9002\u4e2d<\/span><\/p>\n

\u2464\u00a0\u4fe1\u53f7\u5206\u5e03\u4e8e\u6574\u4e2a\u7ec6\u80de<\/span><\/p>\n

\u2465\u00a0\u9002\u5408\u8367\u5149\u663e\u5fae\u955c\u548cHCS\u68c0\u6d4b<\/span><\/p>\n

\u2466\u00a0\u63d0\u4f9b\u591a\u79cd\u53d1\u5c04\u5149\u8c31\u7684Cell-tracker\u793a\u8e2a\u63a2\u9488\uff0c\u9002\u5408\u591a\u6807\u5b9e\u9a8c\uff08\u89c1\u88681\uff09\u3002<\/span><\/p>\n

\u88681. Cell-Tracker\u8367\u5149\u63a2\u9488\u7684\u5149\u8c31\u7279\u5f81<\/b><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n
\u8d27\u53f7<\/strong><\/span><\/td>\n\u540d\u79f0<\/strong><\/span><\/td>\n\u5206\u5b50\u91cf<\/strong><\/span><\/td>\n\u5e38\u7528\u68c0\u6d4b\u901a\u9053<\/strong><\/span><\/td>\nEx\/Em\uff08nm\uff09<\/strong>[1]<\/sup><\/span><\/td>\n<\/tr>\n
MX4107-100UG<\/span><\/td>\nCell-Tracker Green CMFDA<\/span><\/td>\n464.9<\/span><\/td>\nFITC<\/span><\/span><\/td>\n492\/517\u00a0<\/strong>[2]<\/sup><\/span><\/td>\n<\/tr>\n
MX4108-100UG<\/span><\/td>\nCell-Tracker Orange CMTMR<\/span><\/td>\n554.0<\/span><\/td>\nTRITC<\/span><\/td>\n541\/565<\/span><\/td>\n<\/tr>\n
MX4109-100UG<\/span><\/td>\nCell-Tracker Red CMTPX<\/span><\/td>\n686.3<\/span><\/td>\nRhodamine<\/span><\/td>\n577\/602<\/span><\/td>\n<\/tr>\n
MX4110-30UG<\/span><\/td>\nCell-Tracker Deep Red<\/span><\/td>\n698.3<\/span><\/td>\nCy5<\/span><\/td>\n630\/650<\/span><\/td>\n<\/tr>\n
[1] \u6700\u5927\u5438\u6536\u548c\u53d1\u5c04\u8367\u5149\uff0c\u5728\u6c34\u6eb6\u6027\u7f13\u51b2\u6db2\u6216\u7532\u9187\u5185\u786e\u5b9a\u3002\u5728\u7ec6\u80de\u73af\u5883\u5185\u8367\u5149\u503c\u53ef\u80fd\u6709\u4e9b\u8bb8\u53d8\u5316\u3002<\/span><\/td>\n<\/tr>\n
[2] CMFDA\u672c\u8eab\u65e0\u8272\u548c\u65e0\u8367\u5149\uff0c\u76f4\u81f3\u80de\u5185\u916f\u9176\u5207\u5272\u6389\u916f\u5316\u57fa\u56e2\u3002\u916f\u5316\u57fa\u56e2\u7684\u6c34\u89e3\u5f97\u5230\u4ea7\u7269\u7684\u8367\u5149\u7279\u5f81\u5982\u8868\u6240\u8ff0\u3002<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u67d3\u8272\u793a\u4f8b\uff1a<\/b><\/strong><\/span><\/p>\n

\"\"
\nFig 1. <\/strong>Co-culture of CellTracker\u2122 labelled fibroblasts (green) and hESMPs (red) on bilayer membranes of either PHBV\u2013PLA or PHBV\u2013PCL. \uff08Ref.\uff1a<\/strong>Frazer J. Bye et al. Development of bilayer and trilayer nanofibrous\/microfibrous scaffolds for regenerative medicine. Biomater. Sci., 2013, 1, 942-951.<\/span>
\n\"\"
\nFig 2. Measuring O2 exchange in RBCs using single-cell O2-saturating imaging. (C) Wild-type RBCs loaded with a mixture of Deep-Red and Green, showing fluorescence in oxygenated and deoxygenated microstreams.<\/b><\/strong>\u00a0\uff08Ref.\uff1a<\/strong>Sarah L. Richardson, Alzbeta Hulikova, Melanie Proven, Ria Hipkiss, Magbor Akanni, No\u00e9mi B. A. Roy, Pawel Swietach. Single-cell O2 exchange imaging shows that cytoplasmic diffusion is a dominant barrier to efficient gas transport in red blood cells.Proceedings of the National Academy of Sciences May 2020, 117 (18) 10067-10078;\uff09<\/span>
\n\u8ba2\u8d2d\u4fe1\u606f\uff1a<\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n
\u8d27\u53f7<\/strong><\/span><\/td>\n\u4ea7\u54c1\u540d\u79f0<\/strong><\/span><\/td>\n\u89c4\u683c<\/strong><\/span><\/td>\n\u4ef7\u683c\uff08\u5143\uff09<\/strong><\/span><\/td>\n<\/tr>\n
MX4107-100UG<\/span><\/td>\nCell-Tracker Green CMFDA \u6d3b\u7ec6\u80de\u793a\u8e2a\u63a2\u9488\uff08\u7eff\u8272\uff09<\/span><\/td>\n2\u00d750\u03bcg<\/span><\/td>\n980<\/span><\/td>\n<\/tr>\n
MX4108-100UG<\/span><\/td>\nCell-Tracker Orange CMTMR \u6d3b\u7ec6\u80de\u793a\u8e2a\u63a2\u9488\uff08\u6a59\u8272\uff09<\/span><\/td>\n2\u00d750\u03bcg<\/span><\/td>\n1100<\/span><\/td>\n<\/tr>\n
MX4109-100UG<\/span><\/td>\nCell-Tracker Red CMTPX \u6d3b\u7ec6\u80de\u793a\u8e2a\u63a2\u9488\uff08\u7ea2\u8272\uff09<\/span><\/td>\n2\u00d750\u03bcg<\/span><\/td>\n980<\/span><\/td>\n<\/tr>\n
MX4110-30UG<\/span><\/td>\nCell-Tracker Deep Red \u6d3b\u7ec6\u80de\u793a\u8e2a\u63a2\u9488\uff08\u6df1\u7ea2\u8272\uff09<\/span><\/td>\n2\u00d715\u00b5g<\/span><\/td>\n788<\/span><\/td>\n<\/tr>\n
MX4111-1Kit<\/span><\/td>\nCell-Tracker Three-color Trial Kit (Green\/Red\/Deep red) \u6d3b\u7ec6\u80de\u793a\u8e2a\u4e09\u8272\u7ec4\u5408\u8bd5\u5242\u76d2\uff08\u7eff\u8272\/\u7ea2\u8272\/\u6df1\u7ea2\u8272\uff09<\/span><\/td>\n1kit<\/span><\/td>\n1568<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/span><\/p>\n

\u5e38\u89c1\u95ee\u9898\uff08\u9646\u7eed\u52a0\u66f4\u4e2d\u3002\u3002\u3002\u3002\u3002\u3002\uff09<\/strong><\/span><\/p>\n

\u2460<\/strong>\u00a0\u6211\u7684\u5b9e\u9a8c\u9700\u8981\u505a\u7ec6\u80de\u8fc1\u79fb\u7814\u7a76\uff08\u7ea64h\uff09\uff0c\u9700\u8981\u5bf9\u7ec6\u80de\u505a\u8367\u5149\u6807\u8bb0\uff0c\u6709\u63a8\u8350\u7684\u4ea7\u54c1?<\/strong><\/span><\/p>\n

\u6bd4\u5982\uff1a\u53ef\u9009\u62e9\u9499\u9ec4\u7eff\u7d20\uff08Calcein AM\uff09\u548c\u4e8c\u4e59\u9178\u8367\u5149\u7d20\uff08FDA\uff09\u6765\u505a\u6b64\u7528\u9014\u3002\u7531\u4e8e\u8fd9\u4e9b\u67d3\u6599\u4e0d\u4f1a\u63d2\u5165\u6216\u5171\u4ef7\u7ed3\u5408\u5230\u4efb\u4f55\u5176\u5b83\u7ec6\u80de\u6210\u5206\uff0c\u5b83\u4eec\u5728\u80de\u5185\u7684\u6ede\u7559\u65f6\u95f4\u6bd4\u8f83\u77ed\uff0c\u56e0\u4e3a\u67d0\u4e9b\u7ec6\u80de\u7c7b\u578b\u53ef\u80fd\u6781\u5176\u6d3b\u8dc3\u7684\u5c06\u67d3\u6599\u6cf5\u51fa\u7ec6\u80de\u3002\u50cfCell-Tracker\u7cfb\u5217\u67d3\u6599\uff0c\u5305\u542b\u8981\u4e48\u6e29\u548c\u5def\u57fa\u53cd\u5e94\u7684\u6c2f\u7532\u57fa\u57fa\u56e2\u8981\u4e48\u80fa\u57fa\u53cd\u5e94\u7684NHS\u57fa\u56e2\uff0c\u4f7f\u5f97\u63a2\u9488\u5171\u4ef7\u7ed3\u5408\u5230\u7ec6\u80de\u5185\u5bb9\u7269\u4e2d\uff0c\u4f7f\u5f97\u5177\u66f4\u597d\u7684\u6ede\u7559\u6548\u679c\u3002\u4e0d\u7ba1\u662f\u54ea\u79cd\u63a2\u9488\uff0c\u7528\u6237\u6839\u636e\u7ecf\u9a8c\u6765\u786e\u5b9a\u5728\u6240\u7528\u7ec6\u80de\u7c7b\u578b\u5185\u7684\u6ede\u7559\u65f6\u95f4\u3002<\/span><\/p>\n

\u2461<\/strong>\u00a0\u6211\u7684\u5b9e\u9a8c\u7528Calcein AM\u6765\u6807\u8bb0\u7ec6\u80de\uff0c\u4f46\u7b2c\u4e8c\u5929\u6210\u50cf\uff0c\u53d1\u73b0\u6ca1\u6709\u8367\u5149\u4fe1\u53f7\uff0c\u4e3a\u4ec0\u4e48\uff1f<\/strong><\/span><\/p>\n

\u9499\u9ec4\u7eff\u7d20\uff08Calcein AM\uff0cMX3011\uff09\u662f\u826f\u597d\u7684\u7ec6\u80de\u793a\u8e2a\u63a2\u9488\uff0c\u4ee5\u53ca\u5e38\u7528\u7684\u7ec6\u80de\u8d28\u67d3\u6599\u3002\u4f46\uff0c\u4e0d\u4f1a\u4e0e\u80de\u5185\u5185\u542b\u7269\u7ed3\u5408\uff0c\u53ef\u80fd\u51e0\u4e2a\u5c0f\u65f6\u5c31\u4f1a\u88ab\u7ec6\u80de\u6cf5\u51fa\u4f53\u5916\u3002\u9499\u9ec4\u7eff\u7d20\u5728\u6d3b\u7ec6\u80de\u5185\u7684\u6ede\u7559\u65f6\u95f4\u53d6\u51b3\u4e8e\u4e0d\u540c\u7ec6\u80de\u7684\u5185\u5728\u7279\u5f81\u548c\u57f9\u517b\u6761\u4ef6\u3002<\/span><\/p>\n

\u5982\u679c\u8981\u505a\u957f\u671f\u6210\u50cf\uff0c\u66f4\u63a8\u8350\u4f7f\u7528\u5177\u53cd\u5e94\u6027\u7684\u7ec6\u80de\u8d28\u67d3\u6599\u6bd4\u5982\uff1aCFDA SE\uff08MX4104-25MG\uff09\u6216Cell-tracker\u793a\u8e2a\u63a2\u9488\u3002<\/span><\/p>\n

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\u00a0<\/strong><\/span><\/p>\n

 <\/p>\n

\u2014\u2014Written\/Edited by V. Shallan\u3010\u7248\u6743\u5f52\u96c6\u5947\u751f\u7269\/MKBio\u61cb\u5eb7\u6240\u6709\u3011<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

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