描述
FD Tissue Storage Solution™专为延长组织(特别是已用甲醛固定的大脑和脊髓)的保存而设计。该溶液中的组织不会在-20°C下冷冻,并且已被证明可以保留最佳的形态和抗原性。在处理免疫组织化学和/或其他污渍之前,该溶液中的组织可以在-20°C下保存至少5年。
为了获得最佳结果,应将该溶液中存储的组织在0.1 M磷酸盐缓冲液(PB,pH 7.4)中洗涤至少48小时,然后在包含20%蔗糖的0.1 M PB中冷冻保护,然后再进行进一步处理。
FD Tissue Storage Solution™ is specifically designed for the prolonged storage of tissues, especially brains and spinal cords that have been fixed with formaldehyde. Tissues in this solution will not be frozen at -20°C and have been proven to preserve the best morphology and antigenicity. Tissues in this solution may be stored at -20°C for at least 5 years before processing for immunohistochemistry and/or other stains.
For best results, tissues stored in this solution should be washed in 0.1 M phosphate buffer (PB, pH 7.4) for at least 48 hours and then cryoprotected in 0.1 M PB containing 20% sucrose before further processing.