- MX5301-1MG WSP-1 (H2S Probe) 硫化氢荧光探针
Hu YM et.Reactive oxygen species-triggered off-on fluorescence donor for imaging hydrogen sulfide delivery in living cells.Chem. Sci., 2019,10, 7690-7694
WSP-1 (H2S probe) was obtained from Shanghai Maokang Biotechology Co., Ltd.To verify the intracellular ROS-triggered H2S release, a commercially available probe (WSP-1), whose fluorescence can be selectively turned on by H2S,17 was used for the detection of H2S in HeLa cells. As shown in Fig. S17 (images c–e),† the fluorescence of WSP-1 in the green channel rises with increasing the dose of NAB in the presence of H2O2, suggesting the generation of the gradually increased H2S.
- MX4805-1MG Hydroxyphenyl fluorescein (HPF) 羟苯基荧光素
Zhen WY et al.Reductive Surfactant-Assisted One-Step Fabrication of BiOI/BiOIO3 Heterojunction Biophotocatalyst for Enhanced Photodynamic Theranostics Overcoming Tumor Hypoxia.Nanoscale Horiz., 2019, 4, 720-726
Hydroxyphenyl fluorescein (HPF) was purchased from Shanghai Maokang Bio. Co. (Shanghai, China).HPF was used to detect the generation of •OH. Typically, 1 mL BB NCs aqueous solution (80 µg mL -1 ) containing with HPF (1 µM) were irradiated by 650 nm laser (0.5 W cm-2 ), the fluorescence of HPF was detected after 15 min. HPF aqueous solution with laser served as control.
- MX4804-1MG Aminophenyl fluorescein (APF) 氨基苯基荧光素
Wu Q et al.Cascade enzymes within self-assembled hybrid nanogel mimicked neutrophil lysosomes for singlet oxygen elevated cancer therapy.Nat Commun. 2019 Jan 16;10(1):240.
The aminophenyl fluorescein (APF) as the HClO detector were purchased from Shanghai Maokangbio.The further EDT related therapeutic species including the ROS, 1O2 and the intermediate HClO have been detected in tumour tissues of different groups by using the corresponding fluorescent probes, DCFH-DA, SOSG and aminophenyl fluorescein (APF, as HClO detector).
- MS4040-1G Fluorescent Brightener 28 (Calcofluor White M2R) 荧光增白剂28
Yu C et al.Nanoprobe-based force spectroscopy as a versatile platform for probing the mechanical adhesion of bacteria. Nanoscale.2019 Apr 23;11(16):7648-7655. PMID: 30720812
Fluorescent Brightener 28 wasobtained from Maokang Biotechnology (Shanghai, China).For fluorescence staining of extracellularpolymeric substances (EPSs), after the biofilm formation, thePDMS surfaces were incubated with 6 µM SYTO 9 at roomtemperature in the dark for 12 min, followed by a second stain-ing of 0.15 mM fluorescent Brightener 28 for 3 min.
- MX7356-500MG Lywallzyme 真菌溶壁酶
Jin W et al.Importance of a Laccase Gene (Lcc1) in the Development of Ganoderma tsugae.Int J Mol Sci. 2018 Feb 6;19(2). PMID: 29415422
Finally, with gentle agitation,the mycelia (approximately 0.2 g) were incubated for 4–5 h at 31 °C in 2 mL of 20 mg/mL lywallzyme (Shanghai Maokang Biological Technology Co, Ltd., China, Catalog No. MX7365-500MG)containing 0.6 M mannitol for preparing protoplasts. After incubation, these protoplasts were washed free of enzyme mannitol solution and transferred to regeneration medium (RM).
- MZ2103-200UL Brefeldin A (BFA, 5mg/ml)
Zeng C et al.Th17 cells were recruited and accumulated in the cerebrospinal fluid and correlated with the poor prognosis of anti-NMDAR encephalitis. Acta Biochim Biophys Sin (Shanghai).2018 Dec 1;50(12):1266-1273.
Cells were pretreated with 25 ng/ml PMA (Sigma, St Louis, USA) for 5 h at 37°C.At the same time, 10 μg/ml brefeldin (Maokang Biotechnology, Shanghai, China) was added into the cells for the last 4 h of the incubation.
- MX4509-100UG SBFI AM ( Na+ Indicator) 钠离子指示探针
MX4510-100UG PBFI AM ( K+ Indicator) 钾离子指示探针
Tong W et al.Phthalocyanine functionalized poly(glycidyl methacrylate) nano-assemblies for photodynamic inactivation of bacteria.Biomater. Sci., 2019,7, 1905-1918
Sodium-binding benzofuran isophthalate acetoxymethyl ester (SBFI-AM) and Potassium-binding Benzofuran Isophthalate Acetoxymethyl ester (PBFI-AM) were purchased from MKbio. China.
- MP6101-25G BSA, Standard Grade 牛血清白蛋白（标准级别）
Ma MQ et al.Nanocomposite membranes embedded with functionalized MoS2 nanosheets for enhanced interfacialcompatibility and nanofiltration performance.Journal of Membrane Science Volume 591, 1 December 2019, 117316
Bovine serum albumin (BSA, Standard Grade) was acquired from Shanghai Maokang Biotechnology Co., Ltd (China).Polyacrylonitrile ultrafiltration membranes (PAN, MWCO ranging from 10 to 300 kDa) were purchased from Shanghai MegaVision Membrane Engineering & Technology Co., Ltd (China).
- MX3008-5ML Cell Counting Kit (CCK-8)
Liu JY et al.3D printing of biomimetic multi-layered GelMA/nHA scaffold for osteochondral defect repair.Materials & Design Volume 171, 5 June 2019, 107708
Cell counting kit-8 reagent was purchased from Shanghai Maokang Biotechnology Co., Ltd. (China).Cell proliferation on the scaffold was measured with CCK-8 after 1, 3, 5 and 7 days of culture, and cell culture plates were used as blank group. The BMSCs-seeded hydrogels were incubated in DMEM containing 10% CCK-8 protected from light at an incubator of 37 °C and 5% CO2 for 4 h, and measured at 450 nm using a microplate reader.
- MX3012-500T Calcein AM/PI Double Stain Kit 活细胞/死细胞双染试剂盒
Zhang XX et al.Characterization and property of dual-functional Zn-incorporated TiO2 micro-arc oxidation coatings: The influence of current density.Journal of Alloys and Compounds Volume 810, 25 November 2019, 151893
After the formazan was dissolved by dimethyl sulfoxide (DMSO), the optical density (OD) was then determined using enzyme-labeled instrument (iMark, BIO-RAD, US) at the wavelength of 570 nm.To reveal the viability of adherent MC3T3-E1 cells, a Live/Dead Double Staining Kit (MKBio, China).
- MX4801-1KIT Reactive Oxygen Species (ROS) Assay Kit 活性氧检测试剂盒
Wang YW et al.Screening of Duck Tembusu Virus NS3 Interacting Host Proteins and Identification of Its Specific Interplay Domains.Viruses 2019, 11(8), 740;
HEK293 cells were first transfected with pCAGGS and pCAGGS-PRDX1. When PRDX1 was highly expressed for about 24 h, cells were infected with DTMUV and treated for 6 h, whereas the Rosup positive control group was set in parallel.Finally, the probe of DCFH-DA was diluted at 1:1000 and treated at 30 °C for 20 min referring to the ROS Assay Kit (MKBio, Shanghai, China).Cells were then observed under a fluorescent inverted microscope immediately.
- MP1509-25KU Benzonase Nuclease 全能核酸酶（无内毒素）
Raihan O et al.SFRS11 Loss Leads to Aging-Associated Cognitive Decline by Modulating LRP8 and ApoE.Cell Rep. 2019 Jul 2;28(1):78-90.e6.
AAV particles were released from cells with three freeze/thaw cycles, followed by incubation with 50 U/ml benzonase nuclease (MKbio)and 10 U/ml RNase I at 37°C for 30 min, and 0.5% sodium deoxycholate (Sigma) treatment for another 30 min.
- MZ2151-1MG Ionomycin, Free Base 离子霉素（游离酸）
Xu Z et al.Immunomodulatory effects of Rhipicephalus haemaphysaloides serpin RHS2 on host immune responses.Parasit Vectors. 2019 Jul 11;12(1):341.
Ionomycin (Free Base) was purchased from MKbio (Shanghai, China).Recombinant mouse GM-CSF (granulocyte-macrophage colony stimulating factor), IL-4 (interleukin 4) and IL-2 (interleukin 2) were obtained from Peprotech (Rocky Hill, NJ, USA).
- MS4022-100MG Nile Red 尼罗红
Shi Y et al.Increasing the removal of protein-bound uremic toxins by liposome-supported hemodialysis. Artif Organs.2019 May;43(5):490-503.
- MP6308-100UG FITC-UEA-I (FITC labeled Ulex Europaeus Agglutinin I)
MP6013-500UG Human DiI-Ac-LDL
Quan H et al.LncRNA-AK131850 Sponges MiR-93-5p in Newborn and Mature Osteoclasts to Enhance the Secretion of Vascular Endothelial Growth Factor a Promoting Vasculogenesis of Endothelial Progenitor Cells.Cell Physiol Biochem. 2018;46(1):401-417.
After washing, cells were plated on culture dishes at the density of 5×10 6 and cultured with Microvascular Endothelial Cell Growth Medium-2 (EGM-2 MV) BulletKit (Lonza, Walkersville, MD, USA) in a humidified atmosphere of 5% CO2 at 37°C. Two days later, non-adherent cells were removed by washing with PBS twice and the culture medium was replaced every 3 d. After culturing for 1 d, 7 d, 14 d and 28 d, EPCs were identified by optical microscope observation,immunofluorescence for both Dil-Acetylated Low Density Lipoprotein (Dil-Ac- LDL; MaoKang Biotechnology, Shanghai, China) and FITC labeled Ulex Europaeus Agglutinin 1 (FITC-UEA-1; MaoKang Biotechnology, Shanghai, China), and flow cytometry (FACSCalibur Flow Cytometry, BD, Triangle, NC, USA) for PE-Cy7 conjugated anti-mouse CD34 monoclonal antibody.
- MP7501-100T Alkaline Phosphatase Assay Kit (Colorimetric)
Wang CH et al.Colorimetric logic gate for alkaline phosphatase based on copper (II)-based metal-organic frameworks with peroxidase-like activity.Analytica Chimica Acta Volume 1004, 3 April 2018, Pages 74-81
Alkaline Phosphatase Colorimetric Assay Kit using p-nitrophenyl phosphate (pNPP) as aphosphatase substrate was purchased from MKbio Co. Ltd. (Shanghai, China).
- MX2201-1ML Polybrene (10mg/ml)
Dai XJ et al.AHIF promotes glioblastoma progression and radioresistance via exosomes.Int J Oncol. 2019 Jan;54(1):261-270.
The cells were then infected with the same virus titer on the following day with. 8 µg/ml Polybrene (Maokang Co., Shanghai, China). At ~72 h.
18.MS0011-25MG PUROMYCIN DIHYDROCHLORIDE 嘌呤霉素盐酸盐
Zhou J et al.Exosomes Released from Tumor-Associated Macrophages Transfer miRNAs That Induce a Treg/Th17 Cell Imbalance in Epithelial Ovarian Cancer.Cancer Immunol Res. 2018 Dec;6(12):1578-1592. PMID: 30396909